Retrospective investigation of porcine circoviruses in cases of porcine dermatitis and nephropathy syndrome
Cobos, Àlex (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)
Domingo, Mariano (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Pérez, Mónica (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)
Huerta, Eva (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Llorens, Anna Maria (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)
Segalés Coma, Joaquim (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Sibila Vidal, Rosa Marina (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)

Data:	2024
Descripció:	1 pàg.
Resum:	Porcine dermatitis and nephropathy syndrome (PDNS) is a severe condition that affects mainly growing pigs and is considered to be caused by a type III hypersensitivity reaction. Although porcine circovirus 2 (PCV-2) is the antigen linked to this condition, porcine circovirus 3 (PCV-3) has also been proposed to be causally associated with PDNS. Moreover, the initial description of porcine circovirus 4 (PCV-4) also related this novel agent to this clinicopathological entity. Therefore, this retrospective study included a large number of PDNS cases (n = 102) fulfilling specific histologic criteria in search of known porcine circoviruses (PCV-1 to PCV-4) by conventional and/or quantitative PCR (qPCR). All the samples were subjected to PCV-2 immunohistochemistry (IHC) or conventional in situ hybridization (C-ISH), and RNAscope® (R-ISH) was used to study PCV-2 and PCV-3 localization in a subset of the samples. All PDNS cases were PCV-2 positive by qPCR, while 30 of them (29. 4%) yielded PCV-3 qPCR positivity; PCV-2 viral loads were significantly greater than PCV-3 viral loads. All animals were negative for PCV-1 and PCV-4. By C-ISH/IHC, 63 cases (61. 8%) were positive for PCV-2, with low to moderate amounts of antigen. R-ISH demonstrated higher sensitivity, as all studied cases were positive; however, neither PCV-2 nor PCV-3 were consistently found within characteristic PDNS lesions. These results indicate that all PDNS-affected pigs were infected with PCV-2, emphasizing the likelihood that this viral antigen is causally linked to this condition. In contrast, no evidence of the association of PCV-1, PCV-3 or PCV-4 with PDNS was found.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	In situ hybridization ; Porcine circovirus 1 (PCV-1) ; Porcine circovirus 2 (PCV-2) ; Porcine circovirus 3 (PCV-3) ; Porcine circovirus 4 (PCV-4) ; Porcine dermatitis and nephropathy syndrome (PDNS) ; Real-time quantitative polymerase chain reaction (qPCR)
Publicat a:	Veterinary research, Vol. 55 Núm. 1 (2024) , p. 146, ISSN 1297-9716

DOI: 10.1186/s13567-024-01405-8
PMID: 39521988

10 p, 3.2 MB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
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