Heat shock protein 70 kDa (HSP70) is involved in the maintenance of pig sperm function throughout liquid storage at 17 °C
Garriga, Ferran (Universitat de Girona. Departament de Biologia)
Maside, Carolina (Universitat de Girona. Departament de Biologia)
Padilla, Lorena (Universitat de Girona. Departament de Biologia)
Recuero, Sandra (Universitat de Girona. Departament de Biologia)
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Yeste Oliveras, Marc (Institució Catalana de Recerca i Estudis Avançats)

Fecha:	2024
Resumen:	At present, liquid storage is the most efficient method for pig semen preservation. This approach relies upon reducing sperm metabolism, allowing for the maintenance of cell lifespan. In this context, the study of proteins that could protect sperm during liquid storage is of high relevance. The 70 kDa Heat Shock Protein (HSP70) is an anti-apoptotic protein that has been reported to be relevant to sperm survival. Thus, we explored the role of HSP70 during prolonged storage of pig semen at 17 °C. Six semen pools were incubated with YM-1 (0, 0. 05, 0. 1 and 0. 2 μM), an HSP70 inhibitor, and stored at 17 °C for 21 days. On days 0, 4, 10, 14 and 21, sperm quality and function were evaluated through flow cytometry and Computer-Assisted Sperm Analysis (CASA), and HSP70 activity and chromatin condensation were also determined. While inhibition of HSP70 increased progressive motility, Ca 2+ and Reactive Oxygen Species (ROS) levels, and mitochondrial activity during the first 10 days of storage, it had a detrimental effect on sperm motility after 14 and 21 days. In spite of this, sperm viability was not altered. We can conclude that HSP70 contributes to the liquid storage of pig semen because it keeps mitochondrial activity low, which is needed for the maintenance of sperm function.
Ayudas:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00900 Agencia Estatal de Investigación PID2020-113320RB-I00
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	HSP70 ; YM-1 ; Sperm ; Liquid storage ; Flow cytometry ; Pig ; Animal biotechnology ; Biological techniques ; Cell biology ; Developmental biology ; Zoology
Publicado en:	Scientific reports, Vol. 14 (june 2024) , ISSN 2045-2322

DOI: 10.1038/s41598-024-64488-5
PMID: 38862610

11 p, 1.3 MB

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