Impact of Overnight Storage of Human Atrial Myocytes on Intracellular Calcium Homeostasis and Electrophysiological Utility
Aceituno, Cristina (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Revuelta, D. (Partner Site Hamburg/Kiel/Lübeck)
Jiménez-Sábado, Verónica (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Ginel, Antonino (Institut de Recerca Sant Pau)
Molina, C.E. (Partner Site Hamburg/Kiel/Lübeck)
Hove-Madsen, Leif (Institut de Recerca Sant Pau)
Universitat Autònoma de Barcelona

Fecha:	2024
Resumen:	Human atrial myocytes afford an attractive experimental model to investigate mechanisms underlying electrophysiological alterations in cardiovascular disease. However, this model presents limitations, such as the availability of human atrial tissue and a variable yield of myocytes isolation. Therefore, we aimed to determine whether overnight storage can increase the time window where the electrophysiological properties of human atrial myocytes can be determined. To address this issue, human atrial myocytes isolated from patients undergoing cardiac surgery were used for patch-clamp experiments on the day of cell isolation (Day 1) and the following day (Day 2). The shape of the current-voltage (I-V) relationship for the calcium current (I) depended on the access resistance and the cell capacitance, with large cells (>75 pF) requiring a lower access resistance (<15 MΩ) than small cells (<40 pF) to avoid distortion of the I-V curve. Importantly, overnight storage did not significantly affect (1) the I amplitude or properties, (2) sarcoplasmic reticulum calcium homeostasis or (3) the frequency-dependency of the beat-to-beat response. In conclusion, overnight storage of isolated human atrial myocytes at 4 °C does not affect essential features of intracellular calcium homeostasis and, therefore, affords a simple protocol to extend the experimental lifetime of human atrial myocytes.
Ayudas:	Agencia Estatal de Investigación PID2020-116927RB-C21 Agencia Estatal de Investigación PID2023-152610OB-C21
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Beat-to-beat response ; Calcium currents ; Calcium homeostasis ; Human atrial myocyte ; Patch-clamp technique ; Sarcoplasmic reticulum
Publicado en:	Biomolecules, Vol. 14 Núm. 11 (november 2024) , p. 1415, ISSN 2218-273X

DOI: 10.3390/biom14111415
PMID: 39595591

11 p, 2.6 MB

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