Effect of Semen Collection in the Metabolite and Hormonal Content of Rabbit Seminal Plasma
Álvarez-Rodríguez, Manuel (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Espanya))
García-Calvo, Laura (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Gardela Santacruz, Jaume (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Ruiz Conca, Mateo (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Olvera-Maneu, Sergi (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Martín-San Juan, Adrián (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Espanya))
López Béjar, Manel (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Martínez-Pastor, Felipe (Universidad de León)
Vicente-Carrillo, Alejandro (Universidad Complutense de Madrid)

Date:	2025
Abstract:	Blood serum (BS) and seminal plasma (SP) share a plethora of compounds that might present an individual and/or temporal concentration variation. We aimed to determine whether BS and SP concentrations of albumin, calcium, citrate, creatinine, fructose, glucose, lactate, total protein, urea, zinc, cortisol, anti-Müllerian hormone (AMH) and testosterone are related to weekly collections in New Zealand White (NZW) adult rabbit bucks. During a 12-week study, blood samples were obtained at the beginning and the end of the study period, and semen samples were taken twice a week from four NZW adult rabbit bucks, starting at 6-7 months of age. After semen collection, the sperm motility was subjectively assessed, and SP was obtained by centrifugation. BS and SP were evaluated for the above-mentioned metabolites using a Biosystems BA400 automated analyser with commercial-specific kits or enzyme immunoassay (EIA) kits, assessing the effects of the male and time of collection. In addition, a correlation analysis aimed at disclosing associations between parameters in BS and SP was performed. Male effect was not significant for BS, but it was significant for SP albumin, citrate, fructose, glucose, lactate and total protein. In addition, all the correlations in BS were positive, whereas they were more balanced in SP, being close to half of the correlations. In conclusion, variations of some metabolites (albumin, citrate, fructose, glucose, lactate and total protein) appear to be potential biomarkers for rabbit SP, although further studies should test their usefulness for sperm fertility assessment. New Zealand White adult rabbit bucks blood serum (BS) and seminal plasma (SP) share a plethora of compounds that might present an individual and/or temporal concentration variation (12-week study). Our results showed variations of some metabolites (albumin, citrate, fructose, glucose, lactate and total protein) that appear to be potential biomarkers for rabbit SP. However, further studies should test their usefulness for sperm fertility assessment.
Grants:	Ministerio de Ciencia e Innovación CNS2023-144564 Ministerio de Economía y Competitividad IJCI-2015-24380 Agencia Estatal de Investigación PID2019-108320RJ-I00 Agencia Estatal de Investigación PID2022-136561OB-I00 Ministerio de Ciencia e Innovación RYC2020-028615-I
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Biomarker ; Blood serum ; Hormone ; Metabolite ; Rabbit ; Seminal plasma
Published in:	Veterinary Medicine and Science, Vol. 11 (january 2025) , ISSN 2053-1095

DOI: 10.1002/vms3.70236
PMID: 39887575

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