Bull Sperm SWATH-MS-Based Proteomics Reveals Link between High Fertility and Energy Production, Motility Structures, and Sperm-Oocyte Interaction
Gacem, Sabrina (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Castello-Ruiz, María (Hospital Universitari i Politècnic La Fe (València))
Hidalgo, Carlos O. (Servicio Regional de Investigación y Desarrollo Agroalimentario)
Tamargo, Carolina (Servicio Regional de Investigación y Desarrollo Agroalimentario)
Santolaria, Pilar (Universidad de Zaragoza)
Soler, Carles (Universitat de València)
Yániz, Jesús L. (Universidad de Zaragoza)
Silvestre, Miguel A. (Universitat de València)

Date:	2023
Abstract:	The prediction of male or semen fertility potential remains a persistent challenge that has yet to be fully resolved. This work analyzed several in vitro parameters and proteome of spermatozoa in bulls cataloged as high- (HF; n = 5) and low-field (LF; n = 5) fertility after more than a thousand artificial inseminations. Sperm motility was evaluated by computer-assisted sperm analysis. Sperm viability, mitochondrial membrane potential (MMP) and reactive oxygen species (mROS) of spermatozoa were assessed by flow cytometry. Proteome was evaluated by the SWATH-MS procedure. Spermatozoa of HF bulls showed significantly higher total motility than the LF group (41. 4% vs 29. 7%). Rates of healthy sperm (live, high MMP, and low mROS) for HF and LF bull groups were 49% and 43%, respectively (p.
Grants:	Ministerio de Ciencia e Innovación PRTR-C17.I1
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Flow cytometry ; Computer-assisted sperm analysis ; Sperm ; Bull ; Fertility ; Proteome
Published in:	Journal of Proteome Research, Vol. 22 (october 2023) , p. 3607-3624, ISSN 1535-3907

DOI: 10.1021/acs.jproteome.3c00461
PMID: 37782577

18 p, 5.5 MB

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