Bull Sperm SWATH-MS-Based Proteomics Reveals Link between High Fertility and Energy Production, Motility Structures, and Sperm-Oocyte Interaction
Gacem, Sabrina (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Castello-Ruiz, María (Hospital Universitari i Politècnic La Fe (València))
Hidalgo, Carlos O. (Servicio Regional de Investigación y Desarrollo Agroalimentario)
Tamargo, Carolina (Servicio Regional de Investigación y Desarrollo Agroalimentario)
Santolaria, Pilar (Universidad de Zaragoza)
Soler, Carles (Universitat de València)
Yániz, Jesús L. (Universidad de Zaragoza)
Silvestre, Miguel A. (Universitat de València)

Date:	2023
Abstract:	The prediction of male or semen fertility potential remains a persistent challenge that has yet to be fully resolved. This work analyzed several in vitro parameters and proteome of spermatozoa in bulls cataloged as high- (HF; n = 5) and low-field (LF; n = 5) fertility after more than a thousand artificial inseminations. Sperm motility was evaluated by computer-assisted sperm analysis. Sperm viability, mitochondrial membrane potential (MMP) and reactive oxygen species (mROS) of spermatozoa were assessed by flow cytometry. Proteome was evaluated by the SWATH-MS procedure. Spermatozoa of HF bulls showed significantly higher total motility than the LF group (41. 4% vs 29. 7%). Rates of healthy sperm (live, high MMP, and low mROS) for HF and LF bull groups were 49% and 43%, respectively (p > 0. 05). Spermatozoa of HF bulls showed a higher presence of differentially abundant proteins (DAPs) related to both energy production (COX7C), mainly the OXPHOS pathway, and the development of structures linked with the motility process (TPPP2, SSMEM1, and SPAG16). Furthermore, we observed that equatorin (EQTN), together with other DAPs related to the interaction with the oocyte, was overrepresented in HF bull spermatozoa. The biological processes related to protein processing, catabolism, and protein folding were found to be overrepresented in LF bull sperm in which the HSP90AA1 chaperone was identified as the most DAP. Data are available via ProteomeXchange with identifier PXD042286.
Grants:	Ministerio de Ciencia e Innovación PRTR-C17.I1
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Flow cytometry ; Computer-assisted sperm analysis ; Sperm ; Bull ; Fertility ; Proteome
Published in:	Journal of Proteome Research, Vol. 22 (october 2023) , p. 3607-3624, ISSN 1535-3907

DOI: 10.1021/acs.jproteome.3c00461
PMID: 37782577

18 p, 5.5 MB

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