iSuRe-HadCre is an essential tool for effective conditional genetics
Garcia-Gonzalez, Irene (Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC))
Rocha, Susana F. (Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC))
Hamidi, Anahita (Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC))
Garcia-Ortega, Lourdes (Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC))
Regano, Alvaro (Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC))
Sanchez-Muñoz, Maria S. (Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC))
Lytvyn, Mariya (Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC))
Garcia-Cabero, Aroa (Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC))
Roig-Soucase, Sergi (Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC))
Schoofs, Hans (Uppsala University)
Castro, Marco (University of Medicine Berlin. Berlin Institute of Health at Charité)
Sabata Perez, Helena (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)
Potente, Michael (University of Medicine Berlin. Berlin Institute of Health at Charité)
Graupera, Mariona (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)
Makinen, Taija (University of Helsinki)
Benedito, Rui (Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC))

Fecha:	2024
Resumen:	Methods for modifying gene function at high spatiotemporal resolution in mice have revolutionized biomedical research, with Cre-loxP being the most widely used technology. However, the Cre-loxP technology has several drawbacks, including weak activity, leakiness, toxicity, and low reliability of existing Cre-reporters. This is mainly because different genes flanked by loxP sites (floxed) vary widely in their sensitivity to Cre-mediated recombination. Here, we report the generation, validation, and utility of iSuRe-HadCre, a new dual Cre-reporter and deleter mouse line that avoids these drawbacks. iSuRe-HadCre achieves this through a novel inducible dual-recombinase genetic cascade that ensures that cells expressing a fluorescent reporter had only transient Cre activity, that is nonetheless sufficient to effectively delete floxed genes. iSuRe-HadCre worked reliably in all cell types and for the 13 floxed genes tested. This new tool will enable the precise, efficient, and trustworthy analysis of gene function in entire mouse tissues or in single cells.
Ayudas:	Agencia Estatal de Investigación PID2020-120252RB-I00 "la Caixa" Foundation HR19-00120 European Commission 101001814 European Commission 773047 European Commission 814316
Nota:	Altres ajuts: Severo Ochoa Center of Excellence (grant CEX2020-001041-S)
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	Nucleic acids research, Vol. 52, Núm. 13 (July 2024) , ISSN 0305-1048

DOI: 10.1093/nar/gkae472
PMID: 38850155

21 p, 7.1 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP) > Instituto de Investigación contra la Leucemia Josep Carreras
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