Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula
Köster, Pamela C. (Instituto de Salud Carlos III)
Figuereido, Ana M. (Universidade de Aveiro)
Maloney, Jenny G. (United States Department of Agriculture)
Dashti, Alejandro (Instituto de Salud Carlos III)
Bailo, Begoña (Instituto de Salud Carlos III)
Tinoco Torres, Rita (Universidade de Aveiro)
Fonseca, Carlos (Universidade de Aveiro)
Mysterud, Atle (University of Oslo)
Habela, Miguel Ángel (University of Extremadura)
Rivero-Juarez, Antonio (Hospital Universitario Reina Sofía (Còrdova, Espanya))
Vicente, Joaquín (Instituto de Investigación en Recursos Cinegéticos)
Serrano Ferron, Emmanuel (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Arnal, Maria C. (Universidad de Zaragoza. Facultad de Veterinaria)
Fernández de Luco, Daniel (Universidad de Zaragoza)
Armenteros, José A. (Consejería de Infraestructuras, Medio Ambiente y Cambio Climático. Principado de Austurias)
Balseiro, Ana (Universidad de León)
Cardona, Guillermo A. (Livestock Laboratory)
Carvalho, João (Universidade de Aveiro)
Hipólito, Dário (Universidade de Aveiro)
Fernandes, Joana M. (Universidade de Aveiro)
Palmeira, Josman Dantas (Universidade de Aveiro)
Calero-Bernal, Rafael (Universidad Complutense de Madrid)
González-Barrio, David (Instituto de Salud Carlos III)
Santín, Mónica (Agriculture Research Service)
Carmena, David (Instituto de Salud Carlos III)

Fecha:	2024
Resumen:	The ongoing increase in wild boar populations across Europe has fostered human-wildlife conflicts, including the transmission of emerging pathogens with zoonotic importance. Blastocystis is a ubiquitous, faecal-oral transmitted protist that can cause gastrointestinal illnesses and is observed in humans and animals worldwide. The role of wildlife in the epidemiology of Blastocystis is insufficiently understood. Thus, we investigated the occurrence and subtype diversity of Blastocystis in free-ranging wild boars from the Iberian Peninsula using conventional PCR and next-generation amplicon sequencing of a fragment of the ssu RNA gene. A total of 459 wild boar faecal samples were collected across Spain (n = 360) and Portugal (n = 99) between 2014 and 2021. Blastocystis was present in 15. 3% (70/459; 95% CI 12. 1-18. 9) of the wild boars analysed, and its occurrence was significantly higher in Portugal (34. 3%, 34/99; 95% CI 25. 1-44. 6) than in Spain (10. 0%, 36/360; 95% CI 7. 1-13. 6). Seven Blastocystis subtypes (ST5, ST10b, ST13-ST15, ST24b, and ST43) were detected among the surveyed wild boar populations, with greater variability detected in Portuguese samples. ST5 was identified in all the Blastocystis-positive animals, whereas 14. 3% of them harboured ST mixed colonisations. Our results demonstrate that Blastocystis ST5 is particularly adapted to infect wild boars. The additional identification of zoonotic STs reinforces the role of wild boars as spreaders of zoonotic infections with public health significance.
Ayudas:	Instituto de Salud Carlos III PI19CIII/00029 Ministerio de Ciencia e Innovación CP18/00111 Ministerio de Ciencia e Innovación FI20CIII/00002
Nota:	El text de l'article es difon sota Llicència Creative Commons BY
Derechos:	Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Blastocystis ; European wild boar ; Sus scrofa ; Iberian peninsula ; Epidemiology ; NGS ; Subtype diversity wildlife ; Zoonoses ; Spain ; Portugal
Publicado en:	Veterinary research, Vol. 55 (2024) , p. 1-12, ISSN 1297-9716

DOI: 10.1186/s13567-024-01385-9
PMID: 39375799

12 p, 1.3 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Grupo de investigación Wildlife Ecology & Health
Artículos > Artículos de investigación
Artículos > Artículos publicados