Degradation of organophosphate flame retardants by white-rot fungi : Degradation pathways and associated toxicity
Losantos, Diana (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Fernández-Arribas, Julio (Institut de Diagnòstic Ambiental i Estudis de l'Aigua)
Pérez-Trujillo, Míriam (Universitat Autònoma de Barcelona. Servei de Ressonància Magnètica Nuclear)
Eljarrat, Ethel (Institut de Diagnòstic Ambiental i Estudis de l'Aigua)
Sarrà i Adroguer, Montserrat (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Caminal i Saperas, Glòria (Institut de Química Avançada de Catalunya)

Date:	2025
Description:	13 pàg.
Abstract:	The environmental persistence of organophosphate flame retardants (OPFRs) in water is becoming and environmental concern. White Rot Fungi (WRF) have proven its capability to degrade certain OPFRs such as tributyl phosphate (TBP), tris(2-butoxyethyl) phosphate (TBEP), tris(2-chloroethyl) phosphate (TCEP) and tris(2-chloroisopropyl) phosphate (TCPP). Despite this capability, there is limited knowledge about the specific pathways involved in the degradation. In this study, three different WRF were paired with individual OPFRs, and potential transformation products (TPs) were identified by UHPLC-HRMS. Some compounds structures were further validated by NMR. From these data degradation pathways were proposed. TBP was degraded by successive hydroxylation and hydrolysis reactions, with a novel dehydrogenation step suggested. Both TCEP and TCPP underwent oxidative dechlorination, with TCEP experiencing subsequent hydrolysis. Uncommon reductive dehalogenation was also observed. TCPP further underwent hydroxylation and environmentally relevant methylation. TBEP generated numerous TPs, mainly by successive dealkylations, along with hydroxylation. Notably, demethylation in TBEP degradation was proposed for the first time. Additional secondary products were formed through hydroxylation and oxidation of the initial metabolites. Finally, in vivo and in silico toxicity assessments were conducted, identifying certain TPs as potentially toxic.
Grants:	Agencia Estatal de Investigación PID2019-103989RB-100 Ministerio de Ciencia e Innovación TED2021-130639B-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-01008 Ministerio de Ciencia e Innovación PRE2020-095902
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Biodegradation ; Demethylation ; Hydroxylation ; OPFRs ; Transformation products ; Biodegradation, Environmental ; Organophosphates/metabolism ; Water Pollutants, Chemical/metabolism ; Animals ; Flame Retardants/metabolism ; Basidiomycota/metabolism ; Organophosphorus Compounds/metabolism
Published in:	Science of the total environment, Vol. 959 (January 2025) , art. 178260, ISSN 1879-1026

DOI: 10.1016/j.scitotenv.2024.178260
PMID: 39752987

13 p, 4.8 MB

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Articles > Research articles
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