Peripheral and intestinal T lymphocyte subsets in dogs with chronic inflammatory enteropathy
Agulla, Beatriz (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Villaescusa, Alejandra (Universidad Complutense de Madrid. Departamento de Medicina y Cirugía Animal)
Sainz Rodríguez, Ángel (Universidad Complutense de Madrid. Departamento de Medicina y Cirugía Animal)
Díaz-Regañón, David (Universidad Complutense de Madrid. Departamento de Medicina y Cirugía Animal)
Rodríguez Franco, Fernando (Universidad Complutense de Madrid. Departamento de Medicina y Cirugía Animal)
Calleja-Bueno, Lydia (Universidad Alfonso X El Sabio)
Olmeda, Patricia (Universidad Complutense de Madrid. Departamento de Medicina y Cirugía Animal)
García-Sancho, Mercedes (Universidad Complutense de Madrid. Departamento de Medicina y Cirugía Animal)

Fecha:	2024
Resumen:	Dysregulated T lymphocyte response is thought to play a key role in chronic intestinal inflammation (CIE). To evaluate the presence of changes in peripheral and intestinal T lymphocyte subsets and to describe potential immune and inflammatory biomarkers in dogs with CIE. Sixteen healthy dogs and 26 dogs were diagnosed with CIE. Prospective case-control study evaluating peripheral and intestinal T lymphocytes using flow cytometry and inflammatory markers obtained from complete blood cell counts. Dogs with CIE had higher peripheral activated T helper (Th) lymphocytes (87/μL [18-273] CIE, 44/μL [16-162] healthy control (HC, P = . 013) and regulatory T cells (Treg; 108/μL [2-257] CIE, 34/μL [1-114] HC, P = . 004). In the intestinal epithelium, CIE dogs presented lower percentages of Th (4. 55% [1. 75-18. 67] CIE, 8. 77% [3. 79-25. 03] HC, P = . 002), activated Th cells (0. 16% [0. 02-0. 83] CIE, 0. 33% [0. 05-0. 57] HC, P = . 03) and CD4/CD8 ratio (0. 08 [0. 02-0. 39] CIE, 0. 21 [0. 07-0. 85] HC, P = . 003). Conversely, higher percentage of activated T cytotoxic cells (20. 24% [3. 12-77. 12] CIE, 12. 32% [1. 21-39. 22] HC, P = . 04) and interferon-gamma (IFN-γ) producing T lymphocytes (7. 36% [0. 63-55. 83] CIE, 1. 44% [0. 00-10. 56] HC, P = . 01) within the epithelium was observed. In the lamina propria the percentage of Treg lymphocytes was higher (6. 02% [1. 00-21. 48] CIE, 3. 52% [0. 18-10. 52] HC, P = . 02). Systemic and intestinal immune alterations occur in dogs with CIE suggesting that blood IFN-γ producing T lymphocytes and the systemic immune-inflamation index (SII) could potentially serve as biomarkers for the disease.
Nota:	Altres ajuts: Proyecto Santander de la Universidad Complutense de Madrid (PR 26/-16-20319; PR 75/18-21623; PR 87/19-22606).
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Flow cytometry ; IBD ; Intraepithelial lymphocytes ; Lamina propria lymphocytes ; Peripheral blood lymphocytes ; T lymphocytes
Publicado en:	Journal of Veterinary Internal Medicine, Vol. 38 (May/June 2024) , p. 1437-1448, ISSN 1939-1676

DOI: 10.1111/jvim.17036
PMID: 38472110

12 p, 2.7 MB

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