RNA : The Unsuspected Conductor in the Orchestra of Macromolecular Crowding
Zacco, Elsa (Istituto Italiano di Tecnologia. Center for Human Technologies)
Broglia, Laura (Istituto Italiano di Tecnologia. Center for Human Technologies)
Kurihara, Misuzu (Hokkaido University)
Monti, Michele (Istituto Italiano di Tecnologia. Center for Human Technologies)
Gustincich, Stefano (Istituto Italiano di Tecnologia. Center for Human Technologies)
Pastore, Annalisa (UK Dementia Research Institute (London))
Plath, Kathrin (University of California. Department of Biological Chemistry)
Nagakawa, Shinichi (Hokkaido University)
Cerase, Andrea (Università di Pisa. Department of Biology)
Sánchez de Groot, Natalia (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Tartaglia, Gian Gaetano (Institució Catalana de Recerca i Estudis Avançats)

Fecha:	2024
Resumen:	This comprehensive Review delves into the chemical principles governing RNA-mediated crowding events, commonly referred to as granules or biological condensates. We explore the pivotal role played by RNA sequence, structure, and chemical modifications in these processes, uncovering their correlation with crowding phenomena under physiological conditions. Additionally, we investigate instances where crowding deviates from its intended function, leading to pathological consequences. By deepening our understanding of the delicate balance that governs molecular crowding driven by RNA and its implications for cellular homeostasis, we aim to shed light on this intriguing area of research. Our exploration extends to the methodologies employed to decipher the composition and structural intricacies of RNA granules, offering a comprehensive overview of the techniques used to characterize them, including relevant computational approaches. Through two detailed examples highlighting the significance of noncoding RNAs, NEAT1 and XIST, in the formation of phase-separated assemblies and their influence on the cellular landscape, we emphasize their crucial role in cellular organization and function. By elucidating the chemical underpinnings of RNA-mediated molecular crowding, investigating the role of modifications, structures, and composition of RNA granules, and exploring both physiological and aberrant phase separation phenomena, this Review provides a multifaceted understanding of the intriguing world of RNA-mediated biological condensates.
Ayudas:	European Commission 309545 European Commission 855923 European Commission 101098989 European Commission 754490 European Commission 101063903 Ministerio de Ciencia e Innovación RYC2019-026752-I Agencia Estatal de Investigación PID2020-117454RA-I00
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	Chemical Reviews, Vol. 124, Issue 9 (April 2024) , p. 4734-4777, ISSN 1520-6890

DOI: 10.1021/acs.chemrev.3c00575
PMID: 38579177

44 p, 15.6 MB

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