Sequential trypsin and ProAlanase digestions unearth immunological protein biomarkers shrouded by skeletal collagen
Wilkin, Shevan (Griffith University)
Lanigan, Liam T. (University of Copenhagen)
Montes, Nuria (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Sharma, Mukul (National Institute of Research in Tribal Health)
Avanzi, Charlotte (Colorado State University)
Sejdiu, Donikë (University of Zurich)
Majander, Kerttu (University of Zurich, Zurich)
Pfrengle, Saskia (University of Tübingen, Tübingen)
Chiang, Yun (University of Copenhagen)
Kunz, Laura (University of Zurich)
Dittmann, Antje (University of Zurich)
Rühli, Frank (University of Zurich)
Singh, Pushpendra (Model Rural Health Research Unit)
Coll, Maria Fontanals (University of York)
Collins, Matthew J. (University of Cambridge)
Taurozzi, Alberto J. (University of Copenhagen)
Schuenemann, Verena J. (University of Zurich)

Fecha:	2024
Resumen:	This study investigates the efficacy of proteomic analysis of human remains to identify active infections in the past through the detection of pathogens and the host response to infection. We advance leprosy as a case study due to the sequestering of sufferers in leprosaria and the suggestive skeletal lesions that can result from the disease. Here we present a sequential enzyme extraction protocol, using trypsin followed by ProAlanase, to reduce the abundance of collagen peptides and in so doing increase the detection of non-collagenous proteins. Through our study of five individuals from an 11th to 18th century leprosarium, as well as four from a contemporaneous non-leprosy associated cemetery in Barcelona, we show that samples from 2 out of 5 leprosarium individuals extracted with the sequential digestion methodology contain numerous host immune proteins associated with modern leprosy. In contrast, individuals from the non-leprosy associated cemetery and all samples extracted with a trypsin-only protocol did not. Through this study, we advance a palaeoproteomic methodology to gain insights into the health of archaeological individuals and take a step toward a proteomics-based method to study immune responses in past populations.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Biochemistry ; Biochemistry applications ; Biochemistry methods ; Medical biochemistry ; Proteomics
Publicado en:	iScience, Vol. 27, Issue 5 (May 2024) , art. 109663, ISSN 2589-0042

DOI: 10.1016/j.isci.2024.109663
PMID: 38655200

18 p, 4.2 MB

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