Investigating the biochemical response of proton minibeam radiation therapy by means of synchrotron-based infrared microspectroscopy
González Vegas, Roberto (Universitat Autònoma de Barcelona. Departament de Física)
Yousef, Ibraheem (Laboratori de Llum del Sincrotró ALBA)
Seksek, Olivier (French National Centre for Scientific Research)
Ortiz, Ramon (Centre National de la Recherche Scientifique. Université Paris-Saclay)
Bertho, Annaïg (Centre National de la Recherche Scientifique. Université Paris-Saclay)
Juchaux, Marjorie (Centre National de la Recherche Scientifique. Université Paris-Saclay)
Nauraye, Catherine (University Paris-Saclay. Radiation Oncology Department)
Marzi, Ludovic De (University Paris-Saclay. Radiation Oncology Department)
Patriarca, Annalisa (University Paris-Saclay. Radiation Oncology Department)
Prezado, Yolanda (Universidade de Santiago de Compostela. Instituto de Investigación Sanitaria)
Martínez-Rovira, Immaculada (Universitat Autònoma de Barcelona. Departament de Física)

Date:	2024
Abstract:	The biology underlying proton minibeam radiation therapy (pMBRT) is not fully understood. Here we aim to elucidate the biological effects of pMBRT using Fourier Transform Infrared Microspectroscopy (FTIRM). In vitro (CTX-TNA2 astrocytes and F98 glioma rat cell lines) and in vivo (healthy and F98-bearing Fischer rats) irradiations were conducted, with conventional proton radiotherapy and pMBRT. FTIRM measurements were performed at ALBA Synchrotron, and multivariate data analysis methods were employed to assess spectral differences between irradiation configurations and doses. For astrocytes, the spectral regions related to proteins and nucleic acids were highly affected by conventional irradiations and the high-dose regions of pMBRT, suggesting important modifications on these biomolecules. For glioma, pMBRT had a great effect on the nucleic acids and carbohydrates. In animals, conventional radiotherapy had a remarkable impact on the proteins and nucleic acids of healthy rats; analysis of tumour regions in glioma-bearing rats suggested major nucleic acid modifications due to pMBRT.
Grants:	Ministerio de Ciencia, Innovación y Universidades PRE2021-097298 Agencia Estatal de Investigación PID2020-114079RA-I00 Ministerio de Ciencia, Innovación y Universidades RYC2018-024043-I Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00607 European Commission 817908
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Radiotherapy
Published in:	Scientific reports, Vol. 14 (May 2024) , art. 11973, ISSN 2045-2322

DOI: 10.1038/s41598-024-62373-9
PMID: 38796617

11 p, 16.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > The ALBA Synchrotron
Articles > Research articles
Articles > Published articles