Insights into the human cDNA : A descriptive study using library screening in yeast
Alaswad, Z. (University of Science and Technology. Zewail City of Science and Technology)
Attallah, N.E. (University of Science and Technology. Zewail City of Science and Technology)
Aboalazm, B. (University of Science and Technology. Zewail City of Science and Technology)
Elmeslhy, E.S. (University of Science and Technology. Zewail City of Science and Technology)
Mekawy, A.S. (University of Science and Technology. Zewail City of Science and Technology)
Afify, F.A. (University of Science and Technology. Zewail City of Science and Technology)
Mahrous, H.K. (University of Science and Technology. Zewail City of Science and Technology)
Abdalla, A. (University of Science and Technology. Zewail City of Science and Technology)
Rahmoon, M.A. (German University in Cairo)
Mohamed, A.A. (University of Science and Technology. Zewail City of Science and Technology)
Shata, A.H. (University of Science and Technology. Zewail City of Science and Technology)
Mansour, R.H. (Center for Genomics. Helmy Institute for Medical Sciences. Zewail City of Science and Technology)
Aboul-ela, F. (Center for X-Ray Determination of the Structure of Matter. Zewail City of Science and Technology)
Elhadidy, M. (University of Science and Technology. Zewail City of Science and Technology)
Javierre, B. M (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)
El-Khamisy, S.F. (The Institute of Cancer Therapeutics. University of Bradford)
Elserafy, M. (University of Science and Technology. Zewail City of Science and Technology)
Universitat Autònoma de Barcelona

Fecha:	2024
Resumen:	The utilization of human cDNA libraries in yeast genetic screens is an approach that has been used to identify novel gene functions and/or genetic and physical interaction partners through forward genetics using yeast two-hybrid (Y2H) and classical cDNA library screens. Here, we summarize several challenges that have been observed during the implementation of human cDNA library screens in Saccharomyces cerevisiae (budding yeast). Upon the utilization of DNA repair deficient-yeast strains to identify novel genes that rescue the toxic effect of DNA-damage inducing drugs, we have observed a wide range of transcripts that could rescue the strains. However, after several rounds of screening, most of these hits turned out to be false positives, most likely due to spontaneous mutations in the yeast strains that arise as a rescue mechanism due to exposure to toxic DNA damage inducing-drugs. The observed transcripts included mitochondrial hits, non-coding RNAs, truncated cDNAs, and transcription products that resulted from the internal priming of genomic regions. We have also noticed that most cDNA transcripts are not fused with the GAL4 activation domain (GAL4AD), rendering them unsuitable for Y2H screening. Consequently, we utilized Sanger sequencing to screen 282 transcripts obtained from either four different yeast screens or through direct fishing from a human kidney cDNA library. The aim was to gain insights into the different transcription products and to highlight the challenges of cDNA screening approaches in the presence of a significant number of undesired transcription products. In summary, this study describes the challenges encountering human cDNA library screening in yeast as a valuable technique that led to the identification of important molecular mechanisms. The results open research venues to further optimize the process and increase its efficiency.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Genetic screens ; Saccharomyces cerevisiae ; Yeast two-hybrid ; Cdna library screens
Publicado en:	Journal of Genetic Engineering and Biotechnology, Vol. 22 Núm. 4 (december 2024) , p. 100427, ISSN 2090-5920

DOI: 10.1016/j.jgeb.2024.100427
PMID: 39674632

12 p, 3.0 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP) > Instituto de Investigación contra la Leucemia Josep Carreras
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