Multi-Omics Methods Applied to Flower Development
Álvarez Urdiola, Raquel (Centre de Recerca en Agrigenòmica)
Matus, José Tomás (Centre de Recerca en Agrigenòmica)
Riechmann, José Luis (Centre de Recerca en Agrigenòmica)

Imprint:	New York : Humana, 2023
Abstract:	Developmental processes in multicellular organisms depend on the proficiency of cells to orchestrate different gene expression programs. Over the past years, several studies of reproductive organ development have considered genomic analyses of transcription factors and global gene expression changes, modeling complex gene regulatory networks. Nevertheless, the dynamic view of developmental processes requires, as well, the study of the proteome in its expression, complexity, and relationship with the transcriptome. In this chapter, we describe a dual extraction method-for protein and RNA-for the characterization of genome expression at proteome level and its correlation to transcript expression data. We also present a shotgun proteomic procedure (LC-MS/MS) followed by a pipeline for the imputation of missing values in mass spectrometry results.
Grants:	Ministerio de Economía y Competitividad BFU2014-58289-P Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-718 Ministerio de Economía y Competitividad SEV-2015-0533 Agencia Estatal de Investigación PRE2018-084278
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
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Language:	Anglès
Series:	Methods in Molecular Biology ; 2686
Document:	Capítol de llibre ; recerca ; Versió acceptada per publicar
Subject:	Protein extraction ; RNA extraction ; Proteomics ; Transcriptomics ; Flower development ; LC-MS/MS ; Arabidopsis
Published in:	Flower Development: Methods and Protocols, 2023, p. 495-508, ISBN 978-1-0716-3299-4

DOI: 10.1007/978-1-0716-3299-4_23

Postprint 29 p, 899.6 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Books and collections > Book chapters