Gene Expression Analysis by Quantitative Real-Time PCR for Floral Tissues
Álvarez Urdiola, Raquel (Centre de Recerca en Agrigenòmica)
Bustamante, Mariana (Centre de Recerca en Agrigenòmica)
Ribes, Joana (Centre de Recerca en Agrigenòmica)
Riechmann, José Luis (Centre de Recerca en Agrigenòmica)

Imprint:	New York : Humana, 2023
Abstract:	Real-time, or quantitative, reverse transcription polymerase chain reaction (qRT-PCR) is a powerful method for rapid and reliable quantification of mRNA abundance. Although it has not featured prominently in flower development research in the past, the availability of novel techniques for the synchronized induction of flower development, or for the isolation of cell-specific mRNA populations, suggests that detailed quantitative analyses of gene expression over time and in specific tissues and cell types by qRT-PCR will become more widely used. In this chapter, we discuss specific considerations for studying gene expression by using qRT-PCR, such as the identification of suitable reference genes for the experimental set-up used. In addition, we provide protocols for performing qRT-PCR experiments in a multiwell plate format (with the LightCycler 480 system, Roche) and with nanofluidic arrays (BioMark™ system, Fluidigm), which allow the automatic combination of sets of samples with sets of assays, and significantly reduce reaction volume and the number of liquid-handling steps performed during the experiment.
Grants:	Ministerio de Economía y Competitividad BFU2014-58289-P Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-718 Ministerio de Economía y Competitividad SEV-2015-0533 Agencia Estatal de Investigación PRE2018-084278
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Rights:	Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.
Language:	Anglès
Series:	Methods in Molecular Biology ; 2686
Document:	Capítol de llibre ; recerca ; Versió acceptada per publicar
Published in:	Flower Development: Methods and Protocols, 2023, p. 403-428, ISBN 978-1-0716-3299-4

DOI: 10.1007/978-1-0716-3299-4_20

Postprint 43 p, 632.0 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Books and collections > Book chapters