The Administration of Heat Shock Protein-70 Bacterial Homolog (DnaK) Improves the Cumulative Survival and the Expression of Immune-Related Genes in Gnotobiotic Full-Sibling Sea Bass Larvae Challenged with Vibrio anguillarum
Vallejos Vidal, Eva Carolina (Universidad de Santiago de Chile)
Fierro Castro, Maria Camino (Universidad de León)
Santillán-Araneda, María Jesús (Universidad de Santiago de Chile)
Goldstein, Merari (Universidad de Santiago de Chile)
Reyes-Cerpa, Sebastián (Universidad Mayor)
Balasch Alemany, Joan Carles (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Khansari, Ali Reza (University of Gothenburg)
Dierckens, Kristof (Ghent University)
Bossier, Peter (Ghent University)
Tort Bardolet, Lluís (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Reyes Lopez, Felipe Esteban (Universidad de Santiago de Chile)

Fecha:	2025
Resumen:	Heat shock proteins (HSPs), particularly HSP70, play a vital role in fish immune defense against pathogens. The administration of DnaK (bacterial homolog of HSP70) may be a strategy to potentiate the immune response and survival of aquatic organisms. This study evaluates the effect of cells overexpressing DnaK on mortality and immune-related gene expression in gnotobiotic sea bass larvae challenged with Vibrio anguillarum. Larvae were subjected to different treatments: NB (no bacteria), YS0 (E. coli with no plasmid), YS1 (E. coli expressing truncated DnaK), and YS2 (E. coli expressing DnaK), and then infected with V. anguillarum at 7 days post-hatching (dph). Mortality was monitored, and RT-qPCR was used to evaluate immune gene expression at 0, 18, 24, 36, and 120 hpc. While no significant variations were recorded in the non-challenged larvae, constant and sustained mortality was observed in challenged larvae from 60 to 120 hpc. However, lower mortality was observed in the larvae treated with DnaK. DnaK treatment promoted the expression of antimicrobial (hepcidin, transferrin) and chemotaxis genes (ccl4), which was further enhanced after a challenge with V. anguillarum, in conjunction with the modulation of il1β and il-8 at 120 hpc. These findings suggest that DnaK induces a potent innate immune response, improving survival against V. anguillarum and supporting its potential use as a disease-preventive strategy in aquaculture.
Ayudas:	Agencia Estatal de Investigación AGL2016-76069-C2-2-R
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Aquaculture ; HSP70 ; DnaK ; Immune response ; Sea bass larvae ; Vibrio anguillarum
Publicado en:	Animals, Vol. 15, Núm. 11 (June 2025) , art. 1655, ISSN 2076-2615

DOI: 10.3390/ani15111655
PMID: 40509121

25 p, 2.4 MB

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