A single-cell multiomics roadmap of zebrafish spermatogenesis reveals regulatory principles of male germline formation
Burgos-Ruiz, Ana María (Universidad Pablo de Olavide)
Geng, Fan-Suo (Garvan Institute of Medical Research (Sydney, Australia))
Pujol Infantes, Gala (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Sanabria, Estefanía (Universidad Pablo de Olavide)
Brethouwer, Thirsa (Universidad Pablo de Olavide)
Almuedo-Castillo, María (Universidad Pablo de Olavide)
Ruiz-Herrera Moreno, Aurora (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Tena, Juan J. (Universidad Pablo de Olavide)
Bogdanovic, Ozren (University of New South Wales (Sidney, Australia))

Fecha:	2025
Descripción:	27 pàg.
Resumen:	Spermatogenesis is the biological process by which male sperm cells (spermatozoa) are produced in the testes. Beyond facilitating the transmission of genetic information, spermatogenesis also provides a potential framework for inter- and transgenerational inheritance of gene-regulatory states. While extensively studied in mammals, our understanding of spermatogenesis in anamniotes remains limited. Here we present a comprehensive single-cell multiomics resource, combining single-cell RNA sequencing (scRNA-seq) and single-cell chromatin accessibility (scATAC-seq) profiling, with base-resolution DNA methylome (WGBS) analysis of sorted germ cell populations from zebrafish (Danio rerio) testes. We identify the major germ cell types involved in zebrafish spermatogenesis as well as key drivers associated with these transcriptional states. Moreover, we describe localised DNA methylation changes associated with spermatocyte populations, as well as local and global changes in chromatin accessibility leading to chromatin compaction in spermatids. Notably, we identify loci that evade global chromatin compaction, and which remain accessible, suggesting a potential mechanism for the intergenerational transmission of gene-regulatory states. In summary, this high-resolution atlas of zebrafish spermatogenesis provides a valuable resource for studying vertebrate germ cell development and epigenetic inheritance, while offering a robust framework for comparative analyses across diverse models of germ cell biology.
Ayudas:	Agencia Estatal de Investigación PID2021-128358NA-I00 Agencia Estatal de Investigación PID2022-141288NB-I00 Agencia Estatal de Investigación PID2020-113647GA-I00 Agencia Estatal de Investigación PID2020-112557GB-I00 Ministerio de Ciencia e Innovación CEX2020-001088-M Ministerio de Ciencia e Innovación RYC2020-028685-I Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00122
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Spermatogenesis ; Zebrafish ; Epigenetics ; Single-cell Multiomics ; DNA Methylation
Publicado en:	Molecular systems biology, Vol.21, Issue 10 (October 2025) , ISSN 1744-4292

DOI: 10.1038/s44320-025-00157-7

27 p, 13.6 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Instituto de Biotecnología y de Biomedicina (IBB)
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