Exploratory Metabolomic Fingerprinting of Aqueous Humor in Healthy Horses and Donkeys, and in Horses with Ocular Pathologies
Corradini, Ignacio (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
José Cunilleras, Eduard (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Nolis Fañanas, Pau (Universitat Autònoma de Barcelona. Servei de Ressonància Magnètica Nuclear)
López-Murcia, María Mar (Universidad CEU Cardenal Herrera. Departamento de Medicina y Cirugía Animal)
Mayordomo-Febrer, Aloma (Universidad CEU Cardenal Herrera. Departamento de Medicina y Cirugía Animal)

Data:	2025
Resum:	This study aims to generate foundational metabolomic data of aqueous humor (AH) in healthy horses and donkeys, and to investigate potential changes or trends in the metabolomic profile associated with age, sex or ocular pathology in horses. The AH metabolomic fingerprint from 5 donkeys and 35 equine eyes (17 controls, 8 with cataracts, 6 with retinal disease and 4 with anterior chamber disease (ACD)) were analyzed using nuclear magnetic resonance (NMR) spectroscopy. A linear mixed-effects model, with individual horse as a random effect and group as a fixed effect, with multiple testing correction using the Benjamini-Hochberg false discovery rate (FDR) method was used to compare groups. The metabolomic profile of the donkeys and horse's AH is very similar to that of other mammals. Threonine was higher in young horses (p = 0. 04), and creatinine was elevated in males (p = 0. 04). Compared with control groups, dimethyl sulfone was higher in the retina (p < 0. 00) and cataract (p = 0. 05) groups. Arginine (p = 0. 05) and valine (p = 0. 03) were lower in the retina group compared to controls. This study successfully characterized the AH metabolomic profile in healthy horses and donkeys and identified several metabolites that could be associated with ocular pathology, warranting further investigation to determine their potential as biomarkers of ocular disease.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Metabolomics ; Fingerprinting ; Aqueous humor ; Horses ; Donkeys ; Oculomics
Publicat a:	Animals, Vol. 15, Issue 19 (September 2025) , art. 2810, ISSN 2076-2615

DOI: 10.3390/ani15192810
PMID: 41096405

16 p, 1.3 MB

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