Efficacy of oocyte follicular aspiration (OPU) to obtain embryos by intracitoplasmatic sperm injection (ICSI) in the mare and donkey
Casas, Aina (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Catalán, Jaime (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Miró, Jordi (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Additional title:	Biologia de la Reproducció
Date:	2023
Abstract:	The production of embryos in vitro through Ovum Pick Up (OPU) and intracytoplasmic sperm injection (ICSI) are the techniques currently used to produce embryos in vitro in the horse, due to the fact that in vitro fertilization (IVF) so far it offers sub-optimal results. However, although the use of OPU-ICSI can produce commercially acceptable results in the horse and is a technique that is becoming increasingly popular in the equestrian industry, there are no positive results reported so far in the case of the donkey. The aim of this study is to evaluate the efficiency of OPU- ICSI first procedures in horses performed at "Servei de reproducció equina de la Universitat Autònoma de Barcelona" and to compare it with the results obtained in donkeys as a way of improving the conservetion of the Catalan Donkey breed We performed 18 OPUs in mare and 8 in donkeys, from where we obtained oocytes that were maturated. From those matured we performed ICSI and finally assessed blastocyst formation. The results obtained in mares and jennies respectively were 64. 08± 30. 06% and 73. 13±15. 66%. of occyte collection, 68. 51±22. 92% and 65. 31±22. 40% of maturation, 78. 25± 16. 94 % and 62. 26±33. 14% for zigot clavage and 27. 80±16. 87% and 2. 50±7. 07% for blastocist/injected oocyte. o significant differences were observed in collection (P= 0. 7414), oocyte maturation (P= 0. 7705), cleavage (P=0. 202129), but there is significant differences in the final result of blastocyst/injected oocytes (P=0. 0018) In conclusion, the overall results in this study are comparable to those reported elsewhere and demonstrate that OPU can be done in donkeys as performed in mares but ICSI in jennies is still not as efficient as in horses. More research is needed in order to improve this results, taking specially emphasis on ICSI in donkeys. Furthermore, it is the first report of a donkey blastocyst obtained by ICSI, which was only accomplished when oocytes were maturated with preovulatory follicular fluid (PFF).
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Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Biologia de la Reproducció, Vol. 18 (2023) , p. 17-23

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