Valorization of agro-industrial wastes by producing 2-phenylethanol via solid-state fermentation : influence of substrate selection on the process
Martínez-Avila, Oscar (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Muñoz-Torrero, Patricia (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Sanchez, Antoni (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Font i Segura, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Barrena Gómez, Raquel (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Date:	2021
Description:	9 pàg.
Abstract:	2-phenylethanol (2-PE) is a value-added compound widely used in industry due to its rose-like odor and antibacterial properties that can be bioproduced using wastes as raw materials. This study presents the valorization of nine agro-industrial wastes as potential substrates for 2-PE production using an isolated 2-PE producer Pichia kudriavzevii, and the solid-state fermentation (SSF) technology as an alternative approach. The assessed substrates comprised wastes of varied traits such that each of them provided different characteristics to the fermentation. Thus, by using a principal component analysis (PCA), it was possible to identify the most significant characteristics associated with the substrates affecting the 2-PE production. Results show that L-phenylalanine biotransformation was more efficient than de novo synthesis for producing 2-PE. Besides, from the evaluated set, the maximum 2-PE production was achieved with red apple pomace, reaching 1. 7 and 25. 2 mg2PE per gram of used waste through de novo and L-phenylalanine biotransformation, respectively. In that scenario, volumetric productivity and precursor yield were 39. 6 mg2PE L-1h-1 and 0. 69 g2PE per gram of L-phenylalanine added, respectively. From the PCA, it was identified that the reducing sugars content of the substrate, the air-filled porosity of the bed and the L-phenylalanine availability were the most critical parameters (associated with the substrates) influencing the microbial activity and 2-PE production. These results suggest that the desirable traits a solid media needs for promoting 2-PE production via SSF could be reached by using a combination of wastes in a synergistic approach.
Grants:	Ministerio de Economía y Competitividad CTM2015-69513-R
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Solid-state fermentation ; Substrate screening ; Waste valorization ; Aroma compounds ; Rose-like compounds ; Pichia kudriavzevii
Published in:	Waste management (Elmsford), Vol. 121 (February 2021) , p. 403-411, ISSN 1879-2456

DOI: 10.1016/j.wasman.2020.12.036
PMID: 33445113

Postprint 39 p, 1.5 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Engineering > Composting Research Group (GICOM)
Articles > Research articles
Articles > Published articles