Metabolic responses of Quercus ilex seedlings to wounding analysed with nuclear magnetic resonance profiling
Sardans i Galobart, Jordi (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Gargallo-Garriga, Albert (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Pérez-Trujillo, Míriam (Universitat Autònoma de Barcelona. Servei de Ressonància Magnètica Nuclear)
Parella Coll, Teodor (Universitat Autònoma de Barcelona. Servei de Ressonància Magnètica Nuclear)
Seco, R. (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Filella, Iolanda (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Peñuelas, Josep (Centre de Recerca Ecològica i d'Aplicacions Forestals)

Additional title:	Metabolomic responses of Quercus ilex seedlings to wounding simulating herbivory
Date:	2014
Abstract:	Plants defend themselves against herbivory at several levels. One of these is the synthesis of inducible chemical defences. Using NMR metabolomic techniques, we studied the metabolic changes of plant leaves after a wounding treatment simulating herbivore attack in the Mediterranean sclerophyllous tree Quercus ilex. First, an increase in glucose content was observed in wounded plants. There was also an increase in the content of C-rich secondary metabolites such as quinic acid and quercitol, both related to the shikimic acid pathway and linked to defence against biotic stress. There was also a shift in N-storing amino acids, from leucine and isoleucine to asparagine and choline. The observed higher content of asparagine is related to the higher content of choline through serine that was proved to be the precursor of choline. Choline is a general anti-herbivore and pathogen deterrent. The study shows the rapid metabolic response of Q. ilex in defending its leaves, based on a rapid increase in the production of quinic acid, quercitol and choline. The results also confirm the suitability of 1H NMR-based metabolomic profiling studies to detect global metabolome shifts after wounding stress in tree leaves, and therefore its suitability in ecometabolomic studies.
Grants:	Ministerio de Ciencia e Innovación CGL2010-17172 Ministerio de Ciencia e Innovación CTQ2009-08328 Ministerio de Ciencia e Innovación CSD2008-00040 Generalitat de Catalunya 2009/SGR-458 Ministerio de Educación y Ciencia BES-2005-6989
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Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Asparagine ; Choline ; Glucose ; Herbivory ; NMR metabolomics ; Quercitol ; Quinic acid ; Wounding ; SDG 15 - Life on Land ; SDG 13 - Climate Action
Published in:	Plant biology, Vol. 16, Num. 2 (March 2014) , p. 395-403, ISSN 1435-8603

DOI: 10.1111/plb.12032

Postprint 25 p, 402.6 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CREAF (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Articles > Research articles
Articles > Published articles