Biological effects, including oxidative stress and genotoxic damage, of polystyrene nanoparticles in different human hematopoietic cell lines
Rubio Lorente, Laura (Pontificia Universidad Católica Madre y Maestra. Departamento de Ciencias Naturales y Exactas)
Barguilla, Irene (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Domenech, Josefa (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Marcos Dauder, Ricardo (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Hernández Bonilla, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Data:	2020
Resum:	In recent years the terms "micro-/nanoplastics" (MNPLs) have caught special attention due to the increasing levels by which humans are exposed. Among MNPLs, polystyrene nanoparticles (PSNPs) are one of the most represented MNPLs in the environment. These tiny particles may enter into the human body, translocate through human barriers, interacting with blood and lymphatic immune cells, and reaching secondary organs. By using three different human leukocytic cell lines: Raji-B (B-lymphocytes), TK6 (lymphoblasts) and THP-1 (monocytes), we pursued to determine the effects of these PSNPs on the immune cell population. With this aim, the three cell lines were exposed to spherical PSNPs of about 50 nm of diameter and cytotoxicity, cellular uptake, reactive oxygen species (ROS) production, and genotoxicity were assessed at different time-points. Results show differences in all the measured endpoints, among the selected cell lines. Thus, whilst the monocytic THP-1 cells showed the highest particle internalization, no adverse effects were observed in such cells. On the other side, although Raji-B and TK6 showed lesser PSNPs uptake, mild toxicity, ROS production and genotoxicity were detected. These results highlight the importance of the cell line selection when the biological effects of PSNPs are evaluated.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Polystyrene nanoparticles ; ROS ; Genotoxicity ; THP-1 ; Raji-B ; TK6
Publicat a:	Journal of hazardous materials, Vol. 398 (November 2020) , art. 122900, ISSN 1873-3336

DOI: 10.1016/j.jhazmat.2020.122900
PMID: 32464564

Postprint 32 p, 1.1 MB

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