Heparinase I treatment to overcome RNA quantification interference in heparinized liver donor samples : One size fits all?
Dalmau, Mar (Universitat Autònoma de Barcelona)
Charco, Ramon (Vall d'Hebron Institut de Recerca (VHIR))
Bilbao, Itxarone (Universitat Autònoma de Barcelona)
Dopazo, Cristina (Universitat Autònoma de Barcelona)
Caralt, Mireia (Vall d'Hebron Institut de Recerca (VHIR))
Molino, José Andrés (Hospital Universitari Vall d'Hebron)
Gómez Gavara, Concepción (Universitat Autònoma de Barcelona)
Universitat Autònoma de Barcelona. Departament de Cirurgia

Data:	2025
Resum:	MicroRNAs have emerged as potential biomarkers of liver injury during organ transplantation due to their specificity, easy detection and stability in many biofluids. Heparin, which has a well-known inhibitory effect on RT-qPCR based measurements, is commonly used during organ donation. Heparinase I treatment has been used to overcome the inhibiting effect of heparin in RNA RT-qPCR analysis. However, there is a lack of evidence regarding its effective, feasible use improving specific miRNA quantification yield in the liver transplant setting. The aim of this study is to evaluate the effect of heparinase I on miRNA detection levels by RT-qPCR in different samples from liver donors. Prospective, single-centre study including evaluation of liver biopsy, perfusate fluid and serum from deceased organ donors from October 2019 to May 2021. Samples from brain death donors (DBD, n = 4) and donors after circulatory death recovered with abdominal normothermic regional perfusion (DCD n = 4) were analysed for the presence of liver-injury related miRNAs (miR-122 and miR-148a) in the absence or presence of heparinase I (6 IU or 12 IU) to evaluate its effect on miRNA detection levels by RT-qPCR. A subgroup of heparinized serum samples from patients undergoing cardiopulmonary bypass was analysed for validation purposes. The study is registered with ClinicalTrials. gov (), and accrual is complete. The expression of miR-122 relative to reference genes was 44. 5, 16. 8 and 4. 2-fold higher in liver biopsy, perfusates and serum respectively, while miR-148a was 3. 4, 2. 2 and 2. 6-fold higher, without differential expression between donor groups (p.
Ajuts:	Spanish Liver Transplant Society (SETH) N/A
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	PloS one, Vol. 20 (May 2025) , art. e0322899, ISSN 1932-6203

DOI: 10.1371/journal.pone.0322899
PMID: 40354476

15 p, 1.4 MB

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