Improving Sensitivity and Resolution of Dendrimer Identification in MALDI-TOF Mass Spectrometry Using Varied Matrix Combinations
Sanhueza, Claudia (Escuela de Ingeniería, Facultad de Ciencias, Ingeniería y Tecnología, Universidad Mayor, Temuco 4780000, Chile)
Dias, Nathalia Baptista (Scientific and Technological Bioresource Nucleus (BIOREN-UFRO), Universidad de La Frontera, Temuco 4811230, Chile)
Vergara, Daniela (Centro de Excelencia en Medicina Traslacional (CEMT), Facultad de Medicina, Universidad de La Frontera, Temuco 4811230, Chile)
Silva, Lisette (Carrera de Química y Farmacia, Facultad de Medicina, Universidad de La Frontera, Temuco 4811230, Chile)
Chávez Ángel, Emigdio (Catalan Institute of Nanoscience and Nanotechnology, CSIC and BIST, Campus UAB, 08193 Bellaterra, Barcelona, Spain)
Castro-Alvarez, Alejandro (Departamento de Ciencias Preclínicas, Facultad de Medicina, Universidad de La Frontera, Temuco 4811230, Chile)

Data:	2025
Resum:	Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a well-known technique for polymer analysis, particularly for determining the molecular weight and structural details of dendrimers. In this study, we evaluated the performance of various matrices, such as 2',4',6'-trihydroxyacetophenone (THAP), α-cyano-4-hydroxycinnamic acid (HCCA), and sinapinic acid (SA), and their combinations, on the sensitivity and resolution of poly(amidoamine) (PAMAM) dendrimers of different generations (G3. 0, G4. 0, and G5. 0). Our results demonstrated that the combination of HCCA-THAP significantly enhanced spectral resolution and peak intensity compared to individual matrices, particularly for higher-generation dendrimers. This improvement is attributed to the better ionization efficiency achieved with the combined matrices. These findings provide critical insights into optimizing MALDI-TOF MS for the accurate characterization of complex polymers, with potential applications in drug delivery and nanotechnology.
Ajuts:	Chilean National Fund for Scientific and Technological Development (FONDECYT) 3220479 Chilean National Fund for Scientific and Technological Development (FONDECYT) 11200620 NextGenerationEU PRTR-C17.I1 Spanish Research Agency SEV-2017-0706 CERCA Programme/Generalitat de Catalunya SEV-2017-0706
Drets:	Aquesta url de drets no existeix a la base de dades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Dendrimers ; MALDI ; Poly-amidoamine ; Polymer analysis
Publicat a:	Polymers, Vol. 17 (January 2025), art. 219, ISSN 2073-4360

DOI: 10.3390/polym17020219
PMID: 39861291

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