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12 p, 3.5 MB XTEND : Extending the depth of field in cryo soft X-ray tomography / Otón, Joaquín (Centro Nacional de Biotecnología) ; Pereiro, Eva (ALBA Laboratori de Llum de Sincrotró) ; Conesa, José J. (Centro Nacional de Biotecnología) ; Chichón, Francisco J. (Centro Nacional de Biotecnología) ; Luque, Daniel (Centro Nacional de Microbiología (Espanya)) ; Rodríguez, Javier M. (Centro Nacional de Microbiología (Espanya)) ; Pérez-Berná, Ana J. (ALBA Laboratori de Llum de Sincrotró) ; Sorzano, Carlos Oscar S. (Centro Nacional de Biotecnología) ; Klukowska, Joanna (City University of New York. Department of Computer Science, The Graduate Center) ; Herman, Gabor T. (City University of New York. Department of Computer Science, The Graduate Center) ; Vargas, Javier (Centro Nacional de Biotecnología) ; Marabini, Roberto (Universidad Autònoma de Madrid. Escuela Politécnica Superior) ; Carrascosa, José L. (Centro Nacional de Biotecnología) ; Carazo, José M. (Centro Nacional de Biotecnología)
We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. [...]
2017 - 10.1038/srep45808
Scientific reports, Vol. 7 (Abril 2017) , art. 45808  

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