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18 p, 877.3 KB Effects of Different Maturation Systems on Bovine Oocyte Quality, Plasma Membrane Phospholipid Composition and Resistance to Vitrification and Warming / Sprícigo, José F. W. (University of Brasilia. School of Agriculture and Veterinary Medicine) ; Diógenes, Mateus N. (University of Brasilia. School of Agriculture and Veterinary Medicine) ; Leme, Ligiane O. (University of Brasilia. School of Agriculture and Veterinary Medicine) ; Guimarães, Ana L. (University of Brasilia. School of Agriculture and Veterinary Medicine) ; Muterlle, Carolle V. (University of Brasilia. School of Agriculture and Veterinary Medicine) ; Silva, Bianca Damiani Marques (Embrapa Genetic Resources and Biotechnology) ; Solà Oriol, David (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments) ; Pivato, Ivo (University of Brasilia. School of Agriculture and Veterinary Medicine) ; Silva, Luciano Paulino (Embrapa Genetic Resources and Biotechnology) ; Dode, Margot A. N. (Embrapa Genetic Resources and Biotechnology)
The objective of this study was to evaluate the effects of different maturation systems on oocyte resistance after vitrification and on the phospholipid profile of the oocyte plasma membrane (PM). Four different maturation systems were tested: 1) in vitro maturation using immature oocytes aspirated from slaughterhouse ovaries (CONT; n = 136); 2) in vitro maturation using immature oocytes obtained by ovum pick-up (OPU) from unstimulated heifers (IMA; n = 433); 3) in vitro maturation using immature oocytes obtained by OPU from stimulated heifers (FSH; n = 444); and 4) in vivo maturation using oocytes obtained from heifers stimulated 24 hours prior by an injection of GnRH (MII; n = 658). [...]
2015 - 10.1371/journal.pone.0130164
PloS one, Vol. 10 (june 2015)  
Registro completo - Creative Commons

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