Per citar aquest document: http://ddd.uab.cat/record/142498
miRNA Expression Profile Analysis in Kidney of Different Porcine Breeds
Timoneda i Heredia, Oriol (Universitat Autònoma de Barcelona. Departament de Genètica Animal)
Balcells Ortega, Ingrid (Universitat Autònoma de Barcelona. Departament de Genètica Animal)
Núñez Garrote, José Ignacio (Fundació Centre de Recerca en Sanitat Animal)
Egea Sánchez, Raquel (Universitat Autònoma de Barcelona. Departament de Genètica Animal)
Vera Rodríguez, Gonzalo (Universitat Autònoma de Barcelona. Departament de Genètica Animal)
Castelló, Anna (Universitat Autònoma de Barcelona. Departament de Genètica Animal)
Tomas, Anna (Fundació d'Investigació Sanitària de les Illes Balears (FISIB). Programa d'Infecció i Immunitat)
Sánchez Bonastre, Armando (Universitat Autònoma de Barcelona. Departament de Genètica Animal)

Data: 2013
Resum: MicroRNAs (miRNAs) are important post-transcriptional regulators in eukaryotes that target mRNAs repressing their expression. The uncertain process of pig domestication, with different origin focuses, and the selection process that commercial breeds suffered, have generated a wide spectrum of breeds with clear genetic and phenotypic variability. The aim of this work was to define the miRNAs expression profile in kidney of several porcine breeds. Small RNA libraries from kidney were elaborated and high-throughput sequenced with the 454 Genome Sequencer FLX (Roche). Pigs used were classified into three groups: the European origin group (Iberian breed and European Wild Boar ancestor), European commercial breeds (Landrace, Large White and Piétrain breeds) and breeds with Asian origin (Meishan and Vietnamese breeds). A total of 229 miRNAs were described in the pig kidney miRNA profile, including 110 miRNAs out of the 257 previously described pig miRNAs and 119 orthologous miRNAs. The most expressed miRNAs in pig kidney microRNAome were Hsa-miR-200b-3p, Ssc-miR-125b and Ssc-miR-23b. Moreover, 5 novel porcine miRNAs and 3 orthologous miRNAs could be validated through RT-qPCR. miRNA sequence variation was determined in 116 miRNAs, evidencing the presence of isomiRs. 125 miRNAs were differentially expressed between breed groups. The identification of breed-specific miRNAs, which could be potentially associated to certain phenotypes, is becoming a new tool for the study of the genetic variability underlying complex traits and furthermore, it adds a new layer of complexity to the interesting process of pig evolution.
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: article ; recerca ; publishedVersion
Matèria: MicroRNAs ; Sequence databases ; Kidneys ; Animal sexual behavior ; Sequence alignment ; DNA sequence analysis ; Gene expression ; Genomic databases
Publicat a: PLoS one, Vol. 8 Issue 1 (January 2013) , p. e55402, ISSN 1932-6203

DOI: 10.1371/journal.pone.0055402


13 p, 590.3 KB

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