Per citar aquest document: http://ddd.uab.cat/record/142817
Clostridium perfringens Epsilon Toxin Binds to Membrane Lipids and its Cytotoxic Action Depends on Sulfatide.
Gil Giró, Carles (Universitat Autònoma de Barcelona. Institut de Neurociències)
Dorca-Arévalo, Jonatan (Universitat de Barcelona. Departament de Patologia i Terapèutica Experimental)
Blasi, Joan (Universitat de Barcelona. Departament de Patologia i Terapèutica Experimental)

Data: 2015
Resum: Epsilon toxin (Etx) is one of the major lethal toxins produced by Clostridium perfringens types B and D, being the causal agent of fatal enterotoxemia in animals, mainly sheep and goats. Etx is synthesized as a non-active prototoxin form (proEtx) that becomes active upon proteolytic activation. Etx exhibits a cytotoxic effect through the formation of a pore in the plasma membrane of selected cell targets where Etx specifically binds due to the presence of specific receptors. However, the identity and nature of host receptors of Etx remain a matter of controversy. In the present study, the interactions between Etx and membrane lipids from the synaptosome-enriched fraction from rat brain (P2 fraction) and MDCK cell plasma membrane preparations were analyzed. Our findings show that both Etx and proEtx bind to lipids extracted from lipid rafts from the two different models as assessed by protein-lipid overlay assay. Lipid rafts are membrane microdomains enriched in cholesterol and sphingolipids. Binding of proEtx to sulfatide, phosphatidylserine, phosphatidylinositol (3)-phosphate and phosphatidylinositol (5)-phosphate was detected. Removal of the sulphate groups via sulfatase treatment led to a dramatic decrease in Etx-induced cytotoxicity, but not in proEtx-GFP binding to MDCK cells or a significant shift in oligomer formation, pointing to a role of sulfatide in pore formation in rafts but not in toxin binding to the target cell membrane. These results show for the first time the interaction between Etx and membrane lipids from host tissue and point to a major role for sulfatides in C. perfringens epsilon toxin pathophysiology.
Nota: Número d'acord de subvenció MINECO/SAF2011/27566
Nota: Número d'acord de subvenció MINECO/SAF2014/56811-R
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès.
Document: article ; recerca ; publishedVersion
Matèria: Lipids ; Cell binding ; Toxins ; Cell membranes ; Membrane proteins ; Cholesterol ; Multiple sclerosis ; Sulfates
Publicat a: PLoS One, Vol. 10, Issue 10 (October 2015) , p. e0140321, ISSN 1932-6203

DOI: 10.1371/journal.pone.0140321


18 p, 3.3 MB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut de Neurociències (INc)
Articles > Articles de recerca
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 Registre creat el 2015-11-04, darrera modificació el 2016-10-06



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