Clinical response to pandemic h1n1 influenza virus from a fatal and mild case in ferrets
Martínez-Orellana, Pamela (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Martorell Montserrat, Jaume (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Vidaña, Beatriz (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Majó i Masferrer, Natàlia (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Martínez Martínez, Jorge (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Falcón, Ana
Rodríguez-Frandsen, Ariel
Casas, Inmaculada
Pozo, Francisco
García-Migura, Lourdes (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
García-Barreno, Blanca
Melero, Jose A.
Fraile, Lorenzo
Nieto, Amelia
Montoya, Maria

Date:	2015
Abstract:	The majority of pandemic 2009 H1N1 (A(H1N1)pdm09) influenza virus (IV) caused mild symptoms in most infected patients, however, a greater rate of severe disease was observed in healthy young adults and children without co-morbid conditions. The purpose of this work was to study in ferrets the dynamics of infection of two contemporary strains of human A(H1N1)pdm09 IV, one isolated from a patient showing mild disease and the other one from a fatal case. Viral strains isolated from a patient showing mild disease-M (A/CastillaLaMancha/RR5661/2009) or from a fatal case-F (A/CastillaLaMancha/RR5911/2009), both without known comorbid conditions, were inoculated in two groups of ferrets and clinical and pathological conditions were analysed. Mild to severe clinical symptoms were observed in animals from both groups. A clinical score distribution was applied in which ferrets with mild clinical signs were distributed on a non-severe group (NS) and ferrets with severe clinical signs on a severe group (S), regardless of the virus used in the infection. Animals on S showed a significant decrease in body weight compared to animals on NS at 4 to 7 days post-infection (dpi). Clinical progress correlated with histopathological findings. Concentrations of haptoglobin (Hp) and serum amyloid A (SAA) increased on both groups after 2 dpi. Clinically severe infected ferrets showed a stronger antibody response and higher viral titres after infection (p = 0. 001). The severity in the progress of infection was independent from the virus used for infection suggesting that the host immune response was determinant in the outcome of the infection. The diversity observed in ferrets mimicked the variability found in the human population.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Pandemic A(H1N1)pdm09 ; Clinical response ; Ferrets
Published in:	Virology Journal, Vol. 12 (march 2015) , ISSN 1743-422X

DOI: 10.1186/s12985-015-0272-x
PMID: 25888921

10 p, 2.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles