Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
Ternette, Nicola (University of Oxford. The Jenner Institute, Nuffield Department of Medicine)
Yang, Hongbing (University of Oxford. Nuffield Department of Medicine)
Partridge, Thomas (University of Oxford. Nuffield Department of Medicine)
Llano Montero, Anuska (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Cedeño, Samandhy (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Fischer, Roman (University of Oxford. Nuffield Department of Medicine)
Charles, Philip D. (University of Oxford. Nuffield Department of Medicine)
Dudek, Nadine L. (Monash University. Department of Biochemistry and Molecular Biology)
Mothe, Beatriz (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Crespo Casal, Manuel (Hospital Universitari Vall d'Hebron)
Fischer, William M. (Los Alamos National Laboratory. Group T-6, Theoretical Biology)
Korber, Bette T. M. (Los Alamos National Laboratory. Group T-6, Theoretical Biology)
Nielsen, Morten (Technical University of Denmark. Center for Biological Sequence Analysis)
Borrow, Persephone (University of Oxford. Nuffield Department of Medicine)
Purcell, Anthony W. (Monash University. Department of Biochemistry and Molecular Biology)
Brander, Christian (Institució Catalana de Recerca i Estudis Avançats)
Dorrell, Lucy (University of Oxford. The Jenner Institute, Nuffield Department of Medicine)
Kessler, Benedikt M. (University of Oxford. Nuffield Department of Medicine)
Hanke, Tomáš (University of Oxford. The Jenner Institute, Nuffield Department of Medicine)
Universitat Autònoma de Barcelona

Date:	2015
Abstract:	Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4 + T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development.
Grants:	Ministerio de Ciencia e Innovación MTM2008-06747-C02-00 Instituto de Salud Carlos III CM08-00020
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Cytotoxic T cells ; Human immunodeficiency virus type I ; Human leukocyte antigen ; Immunopeptidome ; Mass spectrometry
Published in:	European Journal of Immunology, Vol. 46 (november 2015) , p. 60-69, ISSN 1521-4141

DOI: 10.1002/eji.201545890
PMID: 26467324

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles