Oral microbiome in HIV-associated periodontitis
Noguera-Julian, Marc (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Guillén, Yolanda (Universitat Autònoma de Barcelona)
Peterson, Jessica (Emory University. School of Medicine. Division of Infectious Diseases)
Reznik, David (Emory University. School of Medicine. Division of Infectious Diseases)
Harris, Erica V. (Emory University. Department of Biology)
Joseph, Sandeep J. (Emory University. School of Medicine. Division of Infectious Diseases)
Rivera, Javier (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Kannanganat Sidharthan, Sunil (Emory University. School of Medicine. Division of Infectious Diseases)
Amara, Rama (Emory University. School of Medicine. Division of Infectious Diseases)
Nguyen, Minh Ly (Emory University. School of Medicine. Division of Infectious Diseases)
Mutembo, Simon (Zàmbia. Ministry of Health)
Paredes, Roger (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Read, Timothy D. (Emory University. School of Medicine. Division of Infectious Diseases)
Marconi, Vincent C. (Emory University. School of Medicine. Division of Infectious Diseases)

Date:	2017
Abstract:	Supplemental Digital Content is available in the text HIV-associated periodontal diseases (PD) could serve as a source of chronic inflammation. Here, we sought to characterize the oral microbial signatures of HIV+ and HIV- individuals at different levels of PD severity. This cross-sectional study included both HIV+ and HIV- patients with varying degrees of PD. Two tooth, 2 cheek, and 1 saliva samples were obtained for microbiome analysis. Mothur/SILVADB were used to classify sequences. R/Bioconductor (Vegan, PhyloSeq, and DESeq2) was employed to assess overall microbiome structure differences and differential abundance of bacterial genera between groups. Polychromatic flow cytometry was used to assess immune activation in CD4 and CD8 cell populations. Around 250 cheek, tooth, and saliva samples from 50 participants (40 HIV+ and 10 HIV-) were included. Severity of PD was classified clinically as None/Mild (N), Moderate (M), and Severe (S) with 18 (36%), 16 (32%), and 16 (32%) participants in each category, respectively. Globally, ordination analysis demonstrated clustering by anatomic site (R 2 = 0. 25, P < 0. 001). HIV status and PD severity showed a statistically significant impact on microbiome composition but only accounted for a combined 2% of variation. HIV+ samples were enriched in genera Abiotrophia, Neisseria, Kingella, and unclassified Neisseriaceae and depleted in Leptotrichia and Selenomonas. The Neisseria genus was consistently enriched in HIV+ participants regardless of sampling site and PD level. Immune markers were altered in HIV+ participants but did not show association with the oral microbiome. HIV-associated changes in oral microbiome result in subtle microbial signatures along different stages of PD that are common in independent oral anatomic sites.
Grants:	Instituto de Salud Carlos III RD12-0017-0002
Note:	M.N-J, J.R, Y.G. and R.P acknowledge funds from the Gala contra la SIDA 2013 and 2014 editions, the Nit per la Recerca a la Catalunya Central 2015 edition and the Red de investigación en SIDA.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	HIV ; Neisseria ; Oral microbiome ; Periodontal disease
Published in:	Medicine, Vol. 96, issue 12 (March 2017) , e5821, ISSN 1536-5964

DOI: 10.1097/MD.0000000000005821
PMID: 28328799

9 p, 820.3 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles