Cellulase and xylanase production at pilot scale by solid-state fermentation from coffee husk using specialized consortia : the consistency of the process and the microbial communities involved
Cerda Llanos, Alejandra Patricia (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Mejías, Laura (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Gea Leiva, Teresa (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Sanchez, Antoni (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Date:	2017
Abstract:	Solid state fermentation is a promising technology however rising concerns related to scale up and reproducibility in a productive process. Coffee husk and a specialized inoculum were used in a 4. 5L and then in 50L reactors to assess the reproducibility of a cellulase and hemicellulase production system. Fermentations were consistent in terms of cellulase production and microbial communities. The higher temperatures achieved when operating at 50L generated a shift on the microbial communities and a reduction of nearly 50% on cellulase production at pilot scale. In spite, an overall enzymatic production of 3. 1±0. 5FPUg⁻¹DM and 48±4Ug⁻¹DM for FPase and Xyl activities was obtained, respectively, with low deviation coefficients of 16 and 19% for FPase and Xyl production. Gaseous emissions assessment revealed an emission factor of 2. 6·10⁻³kg volatile organic compounds per Mg of coffee husk and negligible NH₃, CH₄ and N₂O emissions.
Grants:	Ministerio de Economía y Competitividad CTM2015-69513-R
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Subject:	Cellulase ; Coffee husk ; Solid-state fermentation ; Specialized inoculum ; Xylanase
Published in:	Bioresource technology, Vol. 243 (Nov. 2017) , p. 1059-1068, ISSN 0960-8524

DOI: 10.1016/j.biortech.2017.07.076

Preprint 38 p, 515.9 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Engineering > Composting Research Group (GICOM)
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