Cellulase and xylanase production at pilot scale by solid-state fermentation from coffee husk using specialized consortia : the consistency of the process and the microbial communities involved
Cerda Llanos, Alejandra Patricia (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Mejías, Laura (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Gea Leiva, Teresa (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Sanchez, Antoni (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Date: |
2017 |
Abstract: |
Solid state fermentation is a promising technology however rising concerns related to scale up and reproducibility in a productive process. Coffee husk and a specialized inoculum were used in a 4. 5L and then in 50L reactors to assess the reproducibility of a cellulase and hemicellulase production system. Fermentations were consistent in terms of cellulase production and microbial communities. The higher temperatures achieved when operating at 50L generated a shift on the microbial communities and a reduction of nearly 50% on cellulase production at pilot scale. In spite, an overall enzymatic production of 3. 1±0. 5FPUg⁻¹DM and 48±4Ug⁻¹DM for FPase and Xyl activities was obtained, respectively, with low deviation coefficients of 16 and 19% for FPase and Xyl production. Gaseous emissions assessment revealed an emission factor of 2. 6·10⁻³kg volatile organic compounds per Mg of coffee husk and negligible NH₃, CH₄ and N₂O emissions. |
Grants: |
Ministerio de Economía y Competitividad CTM2015-69513-R
|
Rights: |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. |
Language: |
Anglès |
Document: |
Article ; recerca ; Versió sotmesa a revisió |
Subject: |
Cellulase ;
Coffee husk ;
Solid-state fermentation ;
Specialized inoculum ;
Xylanase |
Published in: |
Bioresource technology, Vol. 243 (Nov. 2017) , p. 1059-1068, ISSN 0960-8524 |
DOI: 10.1016/j.biortech.2017.07.076
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Record created 2018-10-04, last modified 2023-10-03