Characterization and proteomic profile of extracellular vesicles from peritoneal dialysis efflux
Carreras-Planella, Laura (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Soler Majoral, Jordi (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Rubio Esteve, Cristina (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Lozano Ramos, Sara Inés (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Franquesa, Marcella (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Bonet Sol, Josep (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Troya Saborido, Maria Isabel (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Borràs i Serres, Francesc Enric (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)

Date:	2017
Abstract:	Peritoneal Dialysis (PD) is considered the best option for a cost-effective mid-term dialysis in patients with Chronic Renal Failure. However, functional failure of the peritoneal membrane (PM) force many patients to stop PD treatment and start haemodialysis. Currently, PM functionality is monitored by the peritoneal equilibration test, a tedious technique that often show changes when the membrane damage is advanced. As in other pathologies, the identification and characterization of extracellular vesicles (EVs) in the peritoneal dialysis efflux (PDE) may represent a non-invasive alternative to identify biomarkers of membrane failure. Using size-exclusion chromatography, we isolated EVs from PDE in a group of patients. Vesicles were characterized by the presence of tetraspanin markers, nanoparticle tracking analysis profile, cryo-electron microscopy and mass spectrometry. Here, we report the isolation and characterization of PDE-EVs. Based on mass spectrometry, we have found a set of well-conserved proteins among patients. Interestingly, the peptide profile also revealed remarkable changes between newly enrolled and longer-treated PD patients. These results are the first step to the identification of PDE-EVs based new markers of PM damage, which could support clinicians in their decision-making in a non-invasive manner.
Grants:	Instituto de Salud Carlos III PI16/00072 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014-SGR-804 Instituto de Salud Carlos III RD16/0009 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014BPB00118
Note:	Altres ajuts: GC/CES07/015
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	PloS one, Vol. 12 Núm. 5 (2017) , p. 1-20, ISSN 1932-6203

DOI: 10.1371/journal.pone.0176987
PMID: 28489901

20 p, 9.7 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles