Stem cells from human cardiac adipose tissue depots show different gene expression and functional capacities
Lambert, Carmen (Institut d'Investigació Biomèdica Sant Pau)
Arderiu, Gemma (Institut d'Investigació Biomèdica Sant Pau)
Béjar, Maria Teresa (Institut d'Investigació Biomèdica Sant Pau)
Crespo Asensio, Javier (Institut d'Investigació Biomèdica Sant Pau)
Baldellou, M. (Institut d'Investigació Biomèdica Sant Pau)
Juan-Babot, Oriol (Institut d'Investigació Biomèdica Sant Pau)
Badimon, Lina (Institut d'Investigació Biomèdica Sant Pau)
Universitat Autònoma de Barcelona

Data:	2019
Resum:	Background: The composition and function of the adipose tissue covering the heart are poorly known. In this study, we have investigated the epicardial adipose tissue (EAT) covering the cardiac ventricular muscle and the EAT covering the left anterior descending artery (LAD) on the human heart, to identify their resident stem cell functional activity. Methods: EAT covering the cardiac ventricular muscle was isolated from the apex (avoiding areas irrigated by major vessels) of the heart (ventricular myocardium adipose tissue (VMAT)) and from the area covering the epicardial arterial sulcus of the LAD (PVAT) in human hearts excised during heart transplant surgery. Adipose stem cells (ASCs) from both adipose tissue depots were immediately isolated and phenotypically characterized by flow cytometry. The different behavior of these ASCs and their released secretome microvesicles (MVs) were investigated by molecular and cellular analysis. Results: ASCs from both VMAT (mASCs) and the PVAT (pASCs) were characterized by the expression of CD105, CD44, CD29, CD90, and CD73. The angiogenic-related genes VEGFA, COL18A1, and TF, as well as the miRNA126-3p and miRNA145-5p, were analyzed in both ASC types. Both ASCs were functionally able to form tube-like structures in three-dimensional basement membrane substrates. Interestingly, pASCs showed a higher level of expression of VEGFA and reduced level of COL18A1 than mASCs. Furthermore, MVs released by mASCs significantly induced human microvascular endothelial cell migration. Conclusion: Our study indicates for the first time that the resident ASCs in human epicardial adipose tissue display a depot-specific angiogenic function. Additionally, we have demonstrated that resident stem cells are able to regulate microvascular endothelial cell function by the release of MVs.
Ajuts:	Ministerio de Ciencia e Innovación SAF2016-76819-R Instituto de Salud Carlos III RD16-00110018 Instituto de Salud Carlos III CB16-11-0041 Instituto de Salud Carlos III P17-01321 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017SGR1480 Ministerio de Economía y Competitividad BES-2014-068269
Nota:	Altres ajuts: This work was supported by grants from FEDER "Una Manera de Hacer Europa"; the Secretary of University and Research. We thank FIC-Fundacion Jesús Serra, Barcelona, Spain, for their continuous support.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Angiogenesis ; Epicardial adipose tissue ; Microvesicles ; Perivascular adipose tissue ; Ventricular myocardium adipose tissue
Publicat a:	Stem cell research & therapy, Vol. 10 Núm. 1 (29 2019) , p. 361, ISSN 1757-6512

DOI: 10.1186/s13287-019-1460-1
PMID: 31783922

12 p, 1.3 MB

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