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Pàgina inicial > Articles > Articles publicats > Dendritic Cells From the Cervical Mucosa Capture and Transfer HIV-1 via Siglec-1 |
Data: | 2019 |
Resum: | Antigen presenting cells from the cervical mucosa are thought to amplify incoming HIV-1 and spread infection systemically without being productively infected. Yet, the molecular mechanism at the cervical mucosa underlying this viral transmission pathway remains unknown. Here we identified a subset of HLA-DR+ CD14+ CD11c+ cervical DCs at the lamina propria of the ectocervix and the endocervix that expressed the type-I interferon inducible lectin Siglec-1 (CD169), which promoted viral uptake. In the cervical biopsy of a viremic HIV-1+ patient, Siglec-1+ cells harbored HIV-1-containing compartments, demonstrating that in vivo, these cells trap viruses. Ex vivo, a type-I interferon antiviral environment enhanced viral capture and trans-infection via Siglec-1. Nonetheless, HIV-1 transfer via cervical DCs was effectively prevented with antibodies against Siglec-1. Our findings contribute to decipher how cervical DCs may boost HIV-1 replication and promote systemic viral spread from the cervical mucosa, and highlight the importance of including inhibitors against Siglec-1 in microbicidal strategies. |
Ajuts: | Ministerio de Economía y Competitividad BES-2014-069931 Ministerio de Economía y Competitividad SAF2015-67334-R Instituto de Salud Carlos III RD16/0025/0007 Instituto de Salud Carlos III CP17/00179 Instituto de Salud Carlos III PI14/01235 Instituto de Salud Carlos III PI17/01470 |
Nota: | Altres ajuts: JM-P and NI-U are supported by the Spanish Secretariat of State of Research, Development and Innovation through grant SAF2016-80033-R. MG is supported by a Marie Curie Career Integration Grant (CIG) from the European Commission and by the Pla estratègic de recerca i innovació en salut (PERIS), from the Catalan government. |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Matèria: | Cervix ; Siglec-1 ; HIV-1 ; Trans-infection ; Myeloid cells |
Publicat a: | Frontiers in immunology, Vol. 10 (april 2019) , p. 825, ISSN 1664-3224 |
14 p, 3.6 MB |