Fine mapping of the peach pollen sterility gene (Ps/ps) and detection of markers for marker-assisted selection
Eduardo, Iban (Centre de Recerca en Agrigenòmica)
Tomás, Carlos de (Centre de Recerca en Agrigenòmica)
Alexiou, Konstantinos G. (Centre de Recerca en Agrigenòmica)
Giovannini, Daniela (Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria. Centro di Ricerca per le Colture di Olive, Frutta e Agrumi (Italia))
Pietrella, M. (Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria. Centro di Ricerca per le Colture di Olive, Frutta e Agrumi (Italia))
Carpenedo, S. (Embrapa Clima Temperado (Brasil))
Bassols Raseira, M. C. (Embrapa Clima Temperado (Brasil))
Batlle, Ignasi (Institut de Recerca i Tecnologia Agroalimentàries)
Cantin, Celia M (Institut de Recerca i Tecnologia Agroalimentàries. Parc Científic i Tecnològic Agroalimentari de Lleida)
Aranzana, Maria José (Centre de Recerca en Agrigenòmica)
Arús i Gorina, Pere (Centre de Recerca en Agrigenòmica)

Data:	2020
Resum:	In peach, pollen sterility, expressed as absence of pollen in the anthers, segregates as an undesired trait in breeding programs. Pollen fertility screening in progenies is not a common practice mainly because it does not affect fruit set since cross-pollination is frequent. It is also a time-consuming activity that coincides with the busy pollination season. Segregation for this trait could be avoided by using molecular markers to identify appropriate parents or male sterile plants for early culling in progenies expected to segregate, thus increasing breeding efficiency. In peach, pollen sterility is determined by a recessive allele in homozygosis of the major gene, Ps/ps, located on chromosome 6. In this work, using a conventional mapping approach combined with bulked segregant analysis using resequencing data, we fine mapped Ps to a region of almost 160 kb and developed molecular markers for marker-assisted breeding. These markers were validated in plant materials from three peach breeding programs, including progenies, advanced selections and cultivars, allowing us to determine that the frequency of the ps allele is high (0. 23) and also to infer the genotypes of a large collection of cultivars and advanced breeding lines.
Ajuts:	Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad AGL2015-68329-R
Nota:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Prunus persica ; Male sterility ; Marker-assisted breeding ; Breeding program
Publicat a:	Molecular Breeding, Vol. 40, Issue 6 (June 2020) , art. 57, ISSN 1380-3743

DOI: 10.1007/s11032-020-01139-3

Postprint 23 p, 964.2 KB

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