Rapid detection of Legionella pneumophila in drinking water, based on filter immunoassay and chronoamperometric measurement
J. Ezenarro, Josune (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Párraga-Niño, Noemí (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Sabriá-Leal, M (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
del Campo, F. Javier (Institut de Microelectrònica de Barcelona)
Muñoz Pascual, Francisco Javier (Institut de Microelectrònica de Barcelona)
Jordi Mas, Jordi (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Uría Moltó, Naroa (Institut de Microelectrònica de Barcelona)

Date:	2020
Abstract:	Legionella is a pathogenic bacterium, ubiquitous in freshwater environments and able to colonise man-made water systems from which it can be transmitted to humans during outbreaks. The prevention of such outbreaks requires a fast, low cost, automated and often portable detection system. In this work, we present a combination of sample concentration, immunoassay detection, and measurement by chronoamperometry. A nitrocellulose microfiltration membrane is used as support for both the water sample concentration and the Legionella immunodetection. The horseradish peroxidase enzymatic label of the antibodies permits using the redox substrate 3,3',5,5'-Tetramethylbenzidine to generate current changes proportional to the bacterial concentration present in drinking water. Carbon screen-printed electrodes are employed in the chronoamperometric measurements. Our system reduces the detection time: from the 10 days required by the conventional culture-based methods, to 2-3 h, which could be crucial to avoid outbreaks. Additionally, the system shows a linear response (R2 value of 0. 99), being able to detect a range of Legionella concentrations between 101 and 104 cfu·mL−1 with a detection limit (LoD) of 4 cfu·mL−1.
Grants:	Ministerio de Ciencia e Innovación CTQ2014-54553-C3-1-R Ministerio de Ciencia e Innovación CTQ2014-54553-C3-2R Ministerio de Ciencia e Innovación RTI2018-101974-B-C22
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Legionella pneumophilla ; Preconcentration ; Immunodetection ; Amperometry
Published in:	Biosensors, Vol. 10, Núm. 9 (September 2020) , art. 102, ISSN 2079-6374

DOI: 10.3390/bios10090102
PMID: 32825468

11 p, 1.3 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles