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Pàgina inicial > Articles > Articles publicats > Stable anchoring of bacteria-based protein nanoparticles for surface enhanced cell guidance |
Data: | 2020 |
Resum: | In tissue engineering, biological, physical, and chemical inputs have to be combined to properly mimic cellular environments and successfully build artificial tissues which can be designed to fulfill different biomedical needs such as the shortage of organ donors or the development of in vitro disease models for drug testing. Inclusion body-like protein nanoparticles (pNPs) can simultaneously provide such physical and biochemical stimuli to cells when attached to surfaces. However, this attachment has only been made by physisorption. To provide a stable anchoring, a covalent binding of lactic acid bacteria (LAB) produced pNPs, which lack the innate pyrogenic impurities of Gram-negative bacteria like Escherichia coli, is presented. The reported micropatterns feature a robust nanoscale topography with an unprecedented mechanical stability. In addition, they are denser and more capable of influencing cell morphology and orientation. The increased stability and the absence of pyrogenic impurities represent a step forward towards the translation of this material to a clinical setting. |
Ajuts: | Ministerio de Economía y Competitividad MAT2016-80826-R Agència de Gestió d'Ajuts Universitaris i de Recerca SGR-918 Agència de Gestió d'Ajuts Universitaris i de Recerca SGR-229 Ministerio de Economía y Competitividad SEV-2015-0496 European Commission 654360 |
Nota: | Altres ajuts: the COST Action CA15126, Fundació La Marató de TV3 Nr. 201812 i ICREA Academia Award |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Matèria: | Artificial tissues ; Biochemical stimuli ; Cellular environment ; Clinical settings ; Gram-negative bacteria ; Lactic acid bacteria ; Nanoscale topography ; Protein nanoparticles |
Publicat a: | Journal of materials chemistry B, Vol. 8, Issue 23 (June 2020) , p. 5080-5088, ISSN 2050-7518 |
9 p, 3.5 MB |