Identification of Promiscuous African Swine Fever Virus T-Cell Determinants Using a Multiple Technical Approach
Bosch Camós, Laia (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Navas, María Jesús (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Pina-Pedrero, Sonia (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Accensi Alemany, Francesc (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Correa-Fiz, Florencia (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Park, Chankyu (Konkuk University. Department of Stem Cells and Regenerative Biology)
Carrascal Pérez, Montserrat (Instituto de Investigaciones Biomédicas de Barcelona)
Domínguez, Javier (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Espanya). Departamento de Biotecnología)
Salas, María Luisa (Centro de Biología Molecular Severo Ochoa)
Nikolin, Veljko (Boehringer Ingelheim Veterinary Research Center)
Collado Miguens, Javier Alonso (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Rodriguez, Fernando (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
López Fernandez, Elisabet (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)

Date:	2021
Abstract:	The development of subunit vaccines against African swine fever (ASF) is mainly hindered by the lack of knowledge regarding the specific ASF virus (ASFV) antigens involved in protection. As a good example, the identity of ASFV-specific CD8+ T-cell determinants remains largely unknown, despite their protective role being established a long time ago. Aiming to identify them, we implemented the IFNγ ELISpot as readout assay, using as effector cells peripheral blood mononuclear cells (PBMCs) from pigs surviving experimental challenge with Georgia2007/1. As stimuli for the ELISpot, ASFV-specific peptides or full-length proteins identified by three complementary strategies were used. In silico prediction of specific CD8+ T-cell epitopes allowed identifying a 19-mer peptide from MGF100-1L, as frequently recognized by surviving pigs. Complementarily, the repertoire of SLA I-bound peptides identified in ASFV-infected porcine alveolar macrophages (PAMs), allowed the characterization of five additional SLA I-restricted ASFV-specific epitopes. Finally, in vitro stimulation studies using fibroblasts transfected with plasmids encoding full-length ASFV proteins, led to the identification of MGF505-7R, A238L and MGF100-1L as promiscuously recognized antigens. Interestingly, each one of these proteins contain individual peptides recognized by surviving pigs. Identification of the same ASFV determinants by means of such different approaches reinforce the results presented here.
Grants:	Ministerio de Ciencia e Innovación AGL2016-78169-C2-1-R Ministerio de Ciencia e Innovación PID2019-107616RB-I00
Note:	Altres ajuts: GC/2015DI037
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	ASFV ; CD8+ T-cells ; Antigen presentation ; IFNγ ELISpot ; Epitope predictions ; Immunopeptidomics ; Promiscuous epitope
Published in:	Vaccines (Basel), Vol. 9 Núm. 1 (2021) , p. 29, ISSN 2076-393X

DOI: 10.3390/vaccines9010029
PMID: 33430316

20 p, 745.4 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles