Stressor-dependant changes in immune parameters in the terrestrial isopod crustacean, porcellio scaber : a focus on nanomaterials
Mayall, Craig (Univerza V Ljubljani. Biotehniški fakulteti)
Dolar, Andraz (Univerza V Ljubljani. Biotehniški fakulteti)
Kokalj, Anita Jemec (Univerza V Ljubljani. Biotehniški fakulteti)
Novak, Sara (Univerza V Ljubljani. Biotehniški fakulteti)
Razinger, Jaka (Agricultural Institute of Slovenia. Plant Protection Department)
Barbero, Francesco (Institut Català de Nanociència i Nanotecnologia)
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)
Drobne, Damjana (Univerza V Ljubljani. Biotehniški fakulteti)

Data:	2021
Resum:	We compared the changes of selected immune parameters of Porcellio scaber to different stressors. The animals were either fed for two weeks with Au nanoparticles (NPs), CeO2 NPs, or Au ions or body-injected with Au NPs, CeO2 NPs, or lipopolysaccharide endotoxin. Contrary to expec-tations, the feeding experiment showed that both NPs caused a significant increase in the total hae-mocyte count (THC). In contrast, the ion-positive control resulted in a significantly decreased THC. Additionally, changes in phenoloxidase (PO)-like activity, haemocyte viability, and nitric oxide (NO) levels seemed to depend on the stressor. Injection experiments also showed stressor-depend-ant changes in measured parameters, such as CeO2 NPs and lipopolysaccharide endotoxin (LPS), caused more significant responses than Au NPs. These results show that feeding and injection of NPs caused an immune response and that the response differed significantly, depending on the exposure route. We did not expect the response to ingested NPs, due to the low exposure concentrations (100 μg/g dry weight food) and a firm gut epithelia, along with a lack of phagocytosis in the digestive system, which would theoretically prevent NPs from crossing the biological barrier. It remains a challenge for future research to reveal what the physiological and ecological significance is for the organism to sense and respond, via the immune system, to ingested foreign material.
Ajuts:	European Commission 671881
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Gold nanoparticles ; Cerium nanoparticles ; Woodlice ; Immune response ; Haemocyte
Publicat a:	Nanomaterials, Vol. 11 Núm. 4 (April 2021) , art. 934, ISSN 2079-4991

DOI: 10.3390/nano11040934
PMID: 33917492

18 p, 13.4 MB

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