Experimental Mycobacterium microti Infection in Bank Voles (Myodes glareolus)
Vidal Barba, Enric (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Burgaya, Judit (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Michelet, Lorraine (Paris-Est University National. Reference Laboratory for Tuberculosis)
Arrieta-Villegas, Claudia (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Cantero, Guillermo (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
de Cruz, Krystel (Paris-Est University National. Reference Laboratory for Tuberculosis)
Tambosco, Jennifer (Paris-Est University National. Reference Laboratory for Tuberculosis)
di Bari, Michele (Istituto Superiore di Sanità. Department of Food Safety, Nutrition and Veterinary Public Health)
Romolo, Nonno (Istituto Superiore di Sanità. Department of Food Safety, Nutrition and Veterinary Public Health)
Boschiroli, Maria Laura (Istituto Superiore di Sanità. Department of Food Safety, Nutrition and Veterinary Public Health)
Pérez de Val, Bernat (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)

Date:	2022
Abstract:	Voles are maintenance hosts of Mycobacterium microti. In line with the goal to eradicate tuberculosis (TB) in livestock, the role of this mycobacteria needs to be assessed since it might interfere with current M. bovis / M. caprae surveillance strategies. To better understand the pathogenesis of TB in voles, an experimental infection model was set up to reproduce M. microti infection in laboratory Bank voles (Myodes glareolus). Two infection routes (intragastric and intraperitoneal) and doses (10 5 and 10 6 CFU/0. 1 mL) were assessed. Voles were culled at different post-infection time points. Serology, histopathology, acid-fast bacilli staining, qPCR, and mycobacterial culture from tissues were performed. In addition, qPCR from feces and oral swabs were conducted to assess bacterial shedding. The model allowed us to faithfully reproduce the disease phenotype described in free-ranging voles and characterize the pathogenesis of the infection. Most animals showed multifocal and diffuse granulomatous lesions in the liver and spleen, respectively. Less frequently, granulomas were observed in lungs, lymph nodes, muscles, and salivary gland. Mycobacterial DNA was detected in feces from a few animals but not in oral swabs. However, one contact uninfected vole seroconverted and showed incipient TB compatible lesions, suggesting horizontal transmission between voles.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Voles ; Wild rodents ; Tuberculosis ; Mycobacterium microti ; Experimental infection
Published in:	Microorganisms, Vol. 10 (january 2022) , ISSN 2076-2607

DOI: 10.3390/microorganisms10010135
PMID: 35056584

15 p, 4.7 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles