Determination of the Synthetic Cannabinoids JWH-122, JWH-210, UR-144 in Oral Fluid of Consumers by GC-MS and Quantification of Parent Compounds and Metabolites by UHPLC-MS/MS
La Maida, Nunzia (University "Politecnica delle Marche" of Ancona)
Pellegrini, Manuela (National Centre on Addiction and Doping, Rome)
Papaseit, Esther (Universitat Autònoma de Barcelona. Departament de Farmacologia, de Terapèutica i de Toxicologia)
Pérez-Mañá, Clara (Universitat Autònoma de Barcelona. Departament de Farmacologia, de Terapèutica i de Toxicologia)
Poyatos, Lourdes (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Ventura, Mireia (Energy Control, Associació Benestar i Desenvolupament)
Galindo, Liliana (Energy Control, Associació Benestar i Desenvolupament)
Busardo, Francesco Paolo (University "Politecnica delle Marche" of Ancona)
Pichini, Simona (National Centre on Addiction and Doping, Rome)
Farre, Magi (Universitat Autònoma de Barcelona. Departament de Farmacologia, de Terapèutica i de Toxicologia)
Marchei, Emilia (National Centre on Addiction and Doping, Istituto Superiore di Sanità, V.Le Regina Elena 299, 00161 Rome, Italy; (S.P.); (E.M.))

Date:	2020
Abstract:	The consumption of synthetic cannabinoids (SCs) has significantly increased in the last decade and the analysis of SCs and their metabolites in human specimens is gaining interest in clinical and forensic toxicology. A pilot study has been carried out using a combination of an initial last generation gas chromatography-mass spectrometry (GC-MS) screening method for the determination of JWH-122, JWH-210, UR-144) in oral fluid (OF) of consumers and an ultra-high performance liquid chromatography high resolution mass spectrometry (UHPLC-HRMS) confirmatory method for the quantification of the parent compounds and their metabolites in the same biological matrix. OF samples were simply liquid-liquid extracted before injecting in both chromatographic systems. The developed methods have been successfully validated and were linear from limit of quantification (LOQ) to 50 ng/mL OF. Recovery of analytes was always higher than 70% and matrix effect always lower than 15% whereas intra-assay and inter-assay precision and accuracy were always better than 16%. After smoking 1 mg JWH-122 or UR-144 and 3 mg JWH-210, maximum concentration of 4. 00-3. 14 ng/mL JWH-122, 8. 10-7. 30 ng/mL JWH-210 ng/mL and 7. 40 and 6. 81 ng/mL UR-144 were measured by GC-MS and UHPLC-HRMS respectively at 20 min after inhalation. Metabolites of JWH 122 and 210 were quantified in OF by UHPLC-HRMS, while that of UR144 was only detectable in traces. Our results provide for the first time information about disposition of these SCs and their metabolites in consumers OF. Last generation GC-MS has proven useful tool to identify and quantify parent SCs whereas UHPLC-HRMS also confirmed the presence of SCs metabolites in the OF of SCs consumers.
Grants:	Ministerio de Economía y Competitividad PI14/00715 Instituto de Salud Carlos III PI17/01962 Ministerio de Economía y Competitividad RD16/0017/0003 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017 SGR 316
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Synthetic cannabinoids ; JWH-122 ; JWH-210 and UR-144 ; Oral fluid ; GC-MS ; UHPLC-HRMS
Published in:	International journal of molecular sciences, Vol. 21 (december 2020) , ISSN 1422-0067

DOI: 10.3390/ijms21249414
PMID: 33321929

14 p, 1.3 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles