Acute pancreatitis promotes the generation of two different exosome populations
Jiménez-Alesanco, A. (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Marcuello, M. (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Pastor-Jiménez, M. (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
López-Puerto, L. (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Bonjoch, Laia (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Gironella, Meritxell (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Carrascal Pérez, Montserrat (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Abian, Joaquin (Institut d'Investigació Biomèdica Sant Pau)
de Madaria, Enrique (Hospital General Universitario de Alicante (Alacant, País Valencià))
Closa Autet, Daniel (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Universitat Autònoma de Barcelona

Date:	2019
Abstract:	Exosomes are small extracellular vesicles that act as intercellular messengers. Previous studies revealed that, during acute pancreatitis, circulating exosomes could reach the alveolar compartment and activate macrophages. However, proteomic analysis suggested that the most likely origin of these exosomes could be the liver instead of the pancreas. The present study aimed to characterize the exosomes released by pancreas to pancreatitis-associated ascitic fluid (PAAF) as well as those circulating in plasma in an experimental model of taurocholate-induced acute pancreatitis in rats. We provide evidence that during acute pancreatitis two different populations of exosomes are generated with relevant differences in cell distribution, protein and microRNA content as well as different implications in their physiological effects. During pancreatitis plasma exosomes, but not PAAF exosomes, are enriched in the inflammatory miR-155 and show low levels of miR-21 and miR-122. Mass spectrometry-based proteomic analysis showed that PAAF exosomes contains 10-30 fold higher loading of histones and ribosomal proteins compared to plasma exosomes. Finally, plasma exosomes have higher pro-inflammatory activity on macrophages than PAAF exosomes. These results confirm the generation of two different populations of exosomes during acute pancreatitis. Deep understanding of their specific functions will be necessary to use them as therapeutic targets at different stages of the disease.
Grants:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-1116 Instituto de Salud Carlos III PI10/01261 Instituto de Salud Carlos III RD12/0021/0033 Instituto de Salud Carlos III PT13/0001
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Extracellular signalling molecules ; Acute pancreatitis
Published in:	Scientific reports, Vol. 9 (december 2019) , ISSN 2045-2322

DOI: 10.1038/s41598-019-56220-5
PMID: 31882721

13 p, 2.8 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles