Proteomics study of human cord blood reticulocyte-derived exosomes
Díaz-Varela, Míriam (Hospital Clínic i Provincial de Barcelona)
de Menezes-Neto, Armando (Hospital Clínic i Provincial de Barcelona)
Perez-Zsolt, Daniel (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Gámez-Valero, Ana (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Seguí-Barber, Joan (Hospital Clínic i Provincial de Barcelona)
Izquierdo Useros, Nuria (Institut Germans Trias i Pujol)
Martinez-Picado, Javier (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Fernández-Becerra, Carmen (Institut Germans Trias i Pujol)
Del Portillo, Hernando A.. (Institut Germans Trias i Pujol)
Universitat Autònoma de Barcelona

Date:	2018
Abstract:	Reticulocyte-derived exosomes (Rex), extracellular vesicles of endocytic origin, were initially discovered as a cargo-disposal mechanism of obsolete proteins in the maturation of reticulocytes into erythrocytes. In this work, we present the first mass spectrometry-based proteomics of human Rex (HuRex). HuRex were isolated from cultures of human reticulocyte-enriched cord blood using different culture conditions and exosome isolation methods. The newly described proteome consists of 367 proteins, most of them related to exosomes as revealed by gene ontology over-representation analysis and include multiple transporters as well as proteins involved in exosome biogenesis and erythrocytic disorders. Immunoelectron microscopy validated the presence of the transferrin receptor. Moreover, functional assays demonstrated active capture of HuRex by mature dendritic cells. As only seven proteins have been previously associated with HuRex, this resource will facilitate studies on the role of human reticulocyte-derived exosomes in normal and pathological conditions affecting erythropoiesis.
Grants:	Agencia Estatal de Investigación SAF2016-80033-R Agencia Estatal de Investigación SAF2016-80655-R Ministerio de Economía y Competitividad SAF2015-71231-REDT
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Scientific reports, Vol. 8 (september 2018) , ISSN 2045-2322

DOI: 10.1038/s41598-018-32386-2
PMID: 30232403

11 p, 3.1 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles