African swine fever virus infection in Classical swine fever subclinically infected wild boars
Cabezón Ponsoda, Óscar (Universitat Autònoma de Barcelona. Servei d'Ecopatologia de Fauna Salvatge)
Muñoz-González, Sara (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Colom Cadena, Andreu (Universitat Autònoma de Barcelona. Servei d'Ecopatologia de Fauna Salvatge)
Pérez-Simó, Marta (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Rosell, Rosa (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Lavín González, Santiago (Universitat Autònoma de Barcelona. Grup de Recerca Wildlife Ecology & Health)
Marco, Ignasi (Universitat Autònoma de Barcelona. Servei d'Ecopatologia de Fauna Salvatge)
Fraile, Lorenzo (Universitat de Lleida. Departament de Producció Animal)
de la Riva, Paloma Martínez (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Espanya). Departamento de Biotecnología)
Rodriguez, Fernando (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Domínguez, Javier (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Espanya). Departamento de Biotecnología)
Ganges, Llilianne (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)

Data:	2017
Resum:	Recently moderate-virulence classical swine fever virus (CSFV) strains have been proven capable of generating postnatal persistent infection (PI), defined by the maintenance of viremia and the inability to generate CSFV-specific immune responses in animals. These animals also showed a type I interferon blockade in the absence of clinical signs. In this study, we assessed the infection generated in 7-week-old CSFV PI wild boars after infection with the African swine fever virus (ASFV). The wild boars were divided in two groups and were infected with ASFV. Group A comprised boars who were CSFV PI in a subclinical form and Group B comprised pestivirus-free wild boars. Some relevant parameters related to CSFV replication and the immune response of CSFV PI animals were studied. Additionally, serum soluble factors such as IFN-α, TNF-α, IL-6, IL-10, IFN-γ and sCD163 were analysed before and after ASFV infection to assess their role in disease progression. After ASFV infection, only the CSFV PI wild boars showed progressive acute haemorrhagic disease; however, the survival rates following ASFV infection was similar in both experimental groups. Notwithstanding, the CSFV RNA load of CSFV PI animals remained unaltered over the study; likewise, the ASFV DNA load detected after infection was similar between groups. Interestingly, systemic type I FN-α and IL-10 levels in sera were almost undetectable in CSFV PI animals, yet detectable in Group B, while detectable levels of IFN-γ were found in both groups. Finally, the flow cytometry analysis showed an increase in myelomonocytic cells (CD172a +) and a decrease in CD4 + T cells in the PBMCs from CSFV PI animals after ASFV infection. Our results showed that the immune response plays a role in the progression of disease in CSFV subclinically infected wild boars after ASFV infection, and the immune response comprised the systemic type I interferon blockade. ASFV does not produce any interference with CSFV replication, or vice versa. ASFV infection could be a trigger factor for the disease progression in CSFV PI animals, as their survival after ASFV was similar to that of the pestivirus-free ASFV-infected group. This fact suggests a high resistance in CSFV PI animals even against a virus like ASFV; this may mean that there are relevant implications for CSF control in endemic countries. The diagnosis of ASFV and CSFV co-infection in endemic countries cannot be ruled out and need to be studied in greater depth.
Ajuts:	Ministerio de Economía y Competitividad AGL2015-66907 Ministerio de Economía y Competitividad AGL2013-48998 Agència de Gestió d'Ajuts Universitaris i de Recerca FI-DGR 2014
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	CSFV ; CSF postnatal persistent infection ; Subclinical CSF ; ASFV ; Wild boars ; Viral load ; Innate immune response ; Adaptive immune response ; Disease
Publicat a:	BMC veterinary research, Vol. 13 (august 2017) , ISSN 1746-6148

DOI: 10.1186/s12917-017-1150-0
PMID: 28764692

12 p, 766.2 KB

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