African swine fever virus infection in Classical swine fever subclinically infected wild boars
Cabezón Ponsoda, Óscar (Universitat Autònoma de Barcelona. Servei d'Ecopatologia de Fauna Salvatge)
Muñoz-González, Sara (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Colom Cadena, Andreu (Universitat Autònoma de Barcelona. Servei d'Ecopatologia de Fauna Salvatge)
Pérez-Simó, Marta (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Rosell, Rosa (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Lavín González, Santiago (Universitat Autònoma de Barcelona)
Marco, Ignasi (Universitat Autònoma de Barcelona. Servei d'Ecopatologia de Fauna Salvatge)
Fraile, Lorenzo (Universitat de Lleida. Departament de Producció Animal)
de la Riva, Paloma Martínez (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Espanya). Departamento de Biotecnología)
Rodriguez, Fernando (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Domínguez, Javier (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Espanya). Departamento de Biotecnología)
Ganges, Llilianne (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)

Data:	2017
Resum:	Recently moderate-virulence classical swine fever virus (CSFV) strains have been proven capable of generating postnatal persistent infection (PI), defined by the maintenance of viremia and the inability to generate CSFV-specific immune responses in animals. These animals also showed a type I interferon blockade in the absence of clinical signs. In this study, we assessed the infection generated in 7-week-old CSFV PI wild boars after infection with the African swine fever virus (ASFV). The wild boars were divided in two groups and were infected with ASFV. Group A comprised boars who were CSFV PI in a subclinical form and Group B comprised pestivirus-free wild boars. Some relevant parameters related to CSFV replication and the immune response of CSFV PI animals were studied. Additionally, serum soluble factors such as IFN-α, TNF-α, IL-6, IL-10, IFN-γ and sCD163 were analysed before and after ASFV infection to assess their role in disease progression. After ASFV infection, only the CSFV PI wild boars showed progressive acute haemorrhagic disease; however, the survival rates following ASFV infection was similar in both experimental groups. Notwithstanding, the CSFV RNA load of CSFV PI animals remained unaltered over the study; likewise, the ASFV DNA load detected after infection was similar between groups. Interestingly, systemic type I FN-α and IL-10 levels in sera were almost undetectable in CSFV PI animals, yet detectable in Group B, while detectable levels of IFN-γ were found in both groups. Finally, the flow cytometry analysis showed an increase in myelomonocytic cells (CD172a +) and a decrease in CD4 + T cells in the PBMCs from CSFV PI animals after ASFV infection. Our results showed that the immune response plays a role in the progression of disease in CSFV subclinically infected wild boars after ASFV infection, and the immune response comprised the systemic type I interferon blockade. ASFV does not produce any interference with CSFV replication, or vice versa. ASFV infection could be a trigger factor for the disease progression in CSFV PI animals, as their survival after ASFV was similar to that of the pestivirus-free ASFV-infected group. This fact suggests a high resistance in CSFV PI animals even against a virus like ASFV; this may mean that there are relevant implications for CSF control in endemic countries. The diagnosis of ASFV and CSFV co-infection in endemic countries cannot be ruled out and need to be studied in greater depth.
Ajuts:	Ministerio de Economía y Competitividad AGL2015-66907 Ministerio de Economía y Competitividad AGL2013-48998 Agència de Gestió d'Ajuts Universitaris i de Recerca FI-DGR2014
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	CSFV ; CSF postnatal persistent infection ; Subclinical CSF ; ASFV ; Wild boars ; Viral load ; Innate immune response ; Adaptive immune response ; Disease
Publicat a:	BMC veterinary research, Vol. 13 (august 2017) , ISSN 1746-6148

DOI: 10.1186/s12917-017-1150-0
PMID: 28764692

12 p, 766.2 KB

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