Rapid Diagnosis of Bloodstream Infections with PCR Followed by Mass Spectrometry
Jordana-Lluch, Elena (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Carolan, Heather E. (Ibis Biosciences, an Abbott company, Carlsbad, California, United States of America)
Giménez, Montserrat (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Sampath, Rangarajan (Ibis Biosciences, an Abbott company, Carlsbad, California, United States of America)
Ecker, David J. (Ibis Biosciences, an Abbott company, Carlsbad, California, United States of America)
Quesada, M. Dolores (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Mòdol, Josep M. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Arméstar, Fernando (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Blyn, Lawrence B. (Ibis Biosciences, an Abbott company, Carlsbad, California, United States of America)
Cummins, Lendell L. (Ibis Biosciences, an Abbott company, Carlsbad, California, United States of America)
Ausina, Vicente (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Martró, Elisa (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia

Date:	2013
Abstract:	Achieving a rapid microbiological diagnosis is crucial for decreasing morbidity and mortality of patients with a bloodstream infection, as it leads to the administration of an appropriate empiric antimicrobial therapy. Molecular methods may offer a rapid alternative to conventional microbiological diagnosis involving blood culture. In this study, the performance of a new technology that uses broad-spectrum PCR coupled with mass spectrometry (PCR/ESI-MS) was evaluated for the detection of microorganisms directly from whole blood. A total of 247 whole blood samples and paired blood cultures were prospectively obtained from 175 patients with a suspicion of sepsis. Both sample types were analyzed using the PCR/ESI-MS technology, and the results were compared with those obtained by conventional identification methods. The overall agreement between conventional methods and PCR/ESI-MS performed in blood culture aliquots was 94. 2% with 96. 8% sensitivity and 98. 5% specificity for the molecular method. When comparing conventional methods with PCR/ESI-MS performed in whole blood specimens, the overall agreement was 77. 1% with 50% sensitivity and 93. 8% specificity for the molecular method. Interestingly, the PCR/ESI-MS technology led to the additional identification of 13 pathogens that were not found by conventional methods. Using the PCR/ESI-MS technology the microbiological diagnosis of bloodstream infections could be anticipated in about half of the patients in our setting, including a small but significant proportion of patients newly diagnosed. Thus, this promising technology could be very useful for the rapid diagnosis of sepsis in combination with traditional methods.
Grants:	Ministerio de Ciencia e Innovación CP09/00044 Ministerio de Ciencia e Innovación PTA2011-5619-T
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	PloS one, Vol. 8 (april 2013) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0062108
PMID: 23626775

9 p, 126.2 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles