Unravelling the Encapsulation of DNA and Other Biomolecules in HAp Microcalcifications of Human Breast Cancer Tissues by Raman Imaging
Marro, Monica (Institut de Ciències Fotòniques)
Rodríguez-Rivero, Anna M. (B. Braun Surgical (Barcelona))
Araujo-Andrade, Cuauhtémoc (Institut de Ciències Fotòniques)
Fernandez Figueras, Maria Teresa (Institut Germans Trias i Pujol)
Pérez-Roca, Laia (Institut Germans Trias i Pujol)
Castellà Fernández, Eva (Institut Germans Trias i Pujol)
Navinés i López, Jordi (Institut Germans Trias i Pujol)
Mariscal, Antonio (Institut Germans Trias i Pujol)
Julian Ibañez, Joan Francesc (Institut Germans Trias i Pujol)
Turon, Pau (B. Braun Surgical (Barcelona))
Loza-Alvarez, Pablo (Institut de Ciències Fotòniques)
Universitat Autònoma de Barcelona

Date:	2021
Abstract:	Microcalcifications are detected through mammography screening and, depending on their morphology and distribution (BI-RADS classification), they can be considered one of the first indicators of suspicious cancer lesions. However, the formation of hydroxyapatite (HAp) calcifications and their relationship with malignancy remains unknown. In this work, we report the most detailed three-dimensional biochemical analysis of breast cancer microcalcifications to date, combining 3D Raman spectroscopy imaging and advanced multivariate analysis in order to investigate in depth the molecular composition of HAp calcifications found in 26 breast cancer tissue biopsies. We demonstrate that DNA has been naturally adsorbed and encapsulated inside HAp microcalcifications. Furthermore, we also show the encapsulation of other relevant biomolecules in HAp calcifications, such as lipids, proteins, cytochrome C and polysaccharides. The demonstration of natural DNA biomineralization, particularly in the tumor microenvironment, represents an unprecedented advance in the field, as it can pave the way to understanding the role of HAp in malignant tissues.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Breast cancer ; DNA mineralization ; Microcalcifications ; Raman spectroscopy ; Tumor microenvironment
Published in:	Cancers, Vol. 13 (may 2021) , ISSN 2072-6694

DOI: 10.3390/cancers13112658
PMID: 34071374

17 p, 3.4 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles